Cloning of the gene encoding the delta subunit of the human T-cell receptor reveals its physical organization within the alpha-subunit locus and its involvement in chromosome translocations in T-cell malignancy.
Cloning of the gene encoding the delta subunit of the human T-cell receptor reveals its physical organization within the alpha-subunit locus and its involvement in chromosome translocations in T-cell malignancy.
复制标题
编码人类 T 细胞受体 δ 亚基的基因的克隆揭示了其在 α 亚基基因座内的物理组织及其与 T 细胞恶性肿瘤中染色体易位的关系。
DOI：
10.1073/pnas.85.11.3933
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发表时间：
1988
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Isobe,M;Russo,G;Haluska,FG;Croce,CM
通过利用"染色体步移"技术，我们获得了包含T细胞受体（TCR）δ链基因的克隆。我们分析了跨越整个J α区延伸TCR α链恒定区5 ′端115个内切酶的克隆，并表明TCR δ链基因位于C α 5 ′端80个内切酶上。TCR δ链基因在表达γ/δ的T细胞系Peer中重排，在表达α/β的T细胞系中缺失。该基因组区域的部分序列分析表明其与先前描述的对应于C δ和J δ片段的cDNA克隆的同一性。此外，我们分析了T细胞白血病的t（8; 14）-（q24; q11）染色体易位，并表明J δ片段在来自该肿瘤的细胞中重排，并可能直接参与易位。因此，新克隆的TCR δ链与携带带14q11的细胞遗传学异常的T细胞恶性肿瘤中染色体易位的发生有关。
By taking advantage of "chromosomal walking" techniques, we have obtained clones that encompass the T-cell receptor (TCR) delta-chain gene. We analyzed clones spanning the entire J alpha region extending 115 kilobases 5' of the TCR alpha-chain constant region and have shown that the TCR delta-chain gene is located over 80 kilobases 5' of C alpha. TCR delta-chain gene is rearranged in the gamma/delta-expressing T-cell line Peer and is deleted in alpha/beta-expressing T-cell lines. Sequence analysis of portions of this genomic region demonstrates its identity with previously described cDNA clones corresponding to the C delta and J delta segments. Furthermore, we have analyzed a t(8;14)-(q24;q11) chromosome translocation from a T-cell leukemia and have shown that the J delta segment is rearranged in cells deriving from this tumor and probably directly involved in the translocation. Thus, the newly cloned TCR delta chain is implicated in the genesis of chromosome translocations in T-cell malignancies carrying cytogenetic abnormalities of band 14q11.