Inhibition of cytolytic T lymphocyte clones reactive with Moloney leukemia virus-associated antigens by monoclonal antibodies: a direct approach to the study of H-2 restriction.
Inhibition of cytolytic T lymphocyte clones reactive with Moloney leukemia virus-associated antigens by monoclonal antibodies: a direct approach to the study of H-2 restriction.
复制标题
通过单克隆抗体抑制与莫洛尼白血病病毒相关抗原反应的溶细胞 T 淋巴细胞克隆：研究 H-2 限制的直接方法。
作者：
A. Weiss;H. Macdonald;J. Cerottini;K. Brunner
通过检测单克隆抗H-2抗体对CTL克隆与靶细胞裂解相互作用的抑制作用，在克隆水平上研究了H-2在细胞溶解T淋巴细胞（CTL）介导裂解同源小鼠Moloney白血病病毒（MoLV）诱导的肿瘤细胞中的抑制作用。通过将有限数量的C57BL/6回归（应答）脾脏细胞放入白细胞-肿瘤细胞微混合培养物中，产生了大量molv特异性CTL克隆。将获得的克隆CTL群体分成5个等份，并在存在（或不存在）3种单克隆抗体中的1种或抗全H-2b单倍型抗血清的情况下检测其裂解活性。其中两种单克隆抗体针对H-2Db，一种针对H-2Kb决定因子。这些试剂的特异性已经通过证明CTL群体对H-2Db和H-2Kb同种异体抗原的裂解抑制而得到验证。在总共51个克隆中，有44个克隆被抗h - 2db（和抗全单倍型）试剂选择性抑制，而抗h - 2kb抗体无抑制作用。其余7个克隆均未被抗h - 2db抗体抑制，3个克隆被抗全单倍型抗血清抑制。这些研究表明，大多数CTL克隆对molv相关抗原的识别仅限于H-2Db区，并且这些克隆的H-2限制存在有限的异质性。
H-2 restriction in cytolytic T lymphocyte (CTL)-mediated lysis of syngeneic murine Moloney leukemia virus (MoLV)-induced tumor cells was studied at the clonal level by testing the inhibitory effect of monoclonal anti-H-2 antibodies on the lytic interaction between CTL clones and target cells. Large numbers of MoLV-specific CTL clones were generated by placing limiting numbers of C57BL/6 regressor (responder) spleen cells into micro-mixed leukocyte-tumor cell cultures. The clonal CTL populations thus obtained were split into 5 aliquots and tested for lytic activity in the presence (or absence) of 1 of 3 monoclonal antibodies or of an anti-whole H-2b haplotype antiserum. Two of the monoclonal antibodies were directed against H-2Db and one against H-2Kb determinants. Specificity of these reagents had been verified by demonstrating inhibition of lysis by CTL populations directed against H-2Db and H-2Kb alloantigens. In 44 of a total of 51 clones tested, results showed selective inhibition by the anti-H-2Db (and the anti-whole haplotype) reagents, and lack of inhibition by the anti-H-2Kb antibody., Of the remaining 7 clones, none was inhibited by the anti-H-2Db antibody, and 3 were inhibited by the anti-whole haplotype antiserum. These studies show that the recognition of MoLV-associated antigens by the majority of CTL clones was restricted to the H-2Db region, and that there exists limited heterogeneity in the H-2 restriction of such clones.