Immunoadsorption to improve gas chromatography/high-resolution mass spectrometry of estradiol-17 beta in plasma.
Immunoadsorption to improve gas chromatography/high-resolution mass spectrometry of estradiol-17 beta in plasma.
复制标题
免疫吸附可改善血浆中雌二醇 17 β 的气相色谱/高分辨率质谱分析。
作者：
S. Gaskell;B. Brownsey
我们描述了一种新的，高选择性的程序测定雌二醇-17 β在血浆中。用微量纤维素偶联的雌二醇-17 β抗血清提取样品。将提取的类固醇转化为双（三甲基甲硅烷基）醚，然后进行气相色谱/高分辨率质谱分析，选择离子监测。通过使用[2 H3]雌二醇-17 β作为内标实现精确定量。
We describe a new, highly selective procedure for the determination of estradiol-17 beta in plasma. Samples are extracted with a micro-cellulose-coupled antiserum to estradiol-17 beta. Conversion of the extracted steroid to the bis(trimethylsilyl) ether is followed by gas chromatography/high-resolution mass spectrometry with selected ion monitoring. Precise quantification is achieved through the use of [2H3]estradiol-17 beta as internal standard.