Ohr Protects Corynebacterium glutamicum against Organic Hydroperoxide Induced Oxidative Stress.
Ohr Protects Corynebacterium glutamicum against Organic Hydroperoxide Induced Oxidative Stress.
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Ohr 保护谷氨酸棒杆菌免受有机氢过氧化物诱导的氧化应激
DOI：
10.1371/journal.pone.0131634
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Shen X
中科院分区：
文献类型：
--
作者：
Si M;Wang J;Xiao X;Guan J;Zhang Y;Ding W;Chaudhry MT;Wang Y;Shen X
Ohr是由有机氢过氧化物抗性（Ohr）基因编码的细菌蛋白，在对有机氢过氧化物的抗性中起关键作用。在本研究中，我们表明，半胱氨酸为基础的巯基依赖OHR的谷氨酸棒杆菌分解有机氢过氧化物比过氧化氢更有效。Ohr的两个Cys残基中的任何一个被Ser残基取代导致活性急剧丧失。电子供体支持C.谷氨酸蛋白主要是脂酰化蛋白（LpdA和Lpd/SucB）。一个Δ ohr突变体表现出对有机氢过氧化物的抗性显著降低，体内活性氧（ROS）显著积累，蛋白质羰基化也显著增强。对过氧化氢的耐受性也降低，但蛋白质羰基化没有大幅度提高。总之，结果明确表明，Ohr是必不可少的介导的有机氢过氧化物抗性的C。谷氨酸。
Ohr, a bacterial protein encoded by the Organic Hydroperoxide Resistance (ohr) gene, plays a critical role in resistance to organic hydroperoxides. In the present study, we show that the Cys-based thiol-dependent Ohr of Corynebacterium glutamicum decomposes organic hydroperoxides more efficiently than hydrogen peroxide. Replacement of either of the two Cys residues of Ohr by a Ser residue resulted in drastic loss of activity. The electron donors supporting regeneration of the peroxidase activity of the oxidized Ohr of C. glutamicum were principally lipoylated proteins (LpdA and Lpd/SucB). A Δohr mutant exhibited significantly decreased resistance to organic hydroperoxides and marked accumulation of reactive oxygen species (ROS) in vivo; protein carbonylation was also enhanced notably. The resistance to hydrogen peroxide also decreased, but protein carbonylation did not rise to any great extent. Together, the results unequivocally show that Ohr is essential for mediation of organic hydroperoxide resistance by C. glutamicum.
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影响因子：
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