Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits.
Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits.
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DOI：
10.3389/fimmu.2017.00354
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发表时间：
2017
影响因子：
7.3
通讯作者：
Chai T
中科院分区：
文献类型：
--
作者：
Guo M;Wu F;Hao G;Qi Q;Li R;Li N;Wei L;Chai T
益生菌如乳酸杆菌和双歧杆菌已成功地用于促进生长和预防疾病。以往的研究表明，枯草芽孢杆菌（B. subtilis）是一种潜在的动物益生菌。在本研究中，180 B.从土壤中分离枯草芽孢杆菌，鉴定，并在体外进行研究。此外，5个B。枯草芽孢杆菌，并混合，以研究其对兔的生长性能，免疫反应，肠道菌群和抗病性的影响。兔饲料中含有106 CFU g−1混合B。枯草杆菌的生长性能最好，血清IgG和伊加水平显著高于对照组（P < 0.05）。此外，乳制品与B。枯草杆菌能促进肠道植物群平衡。与对照组相比，主要的促炎因子和β-防御素表达上调。经7周饲养后，用B.枯草芽孢杆菌感染后的细胞数显著高于大肠杆菌感染后的对照组。同时，在日粮中添加B可提高β-防御素的表达，降低心脏和盲肠内容物的细菌含量。与对照组相比，总之，膳食补充B。枯草芽孢杆菌能提高家兔的生长性能、肠道稳态和免疫器官指数，增强天然免疫应答和抗病能力。结果表明，B.枯草芽孢杆菌可能是一个有趣的新的治疗策略，以加强先天防御机制。
Probiotics such as Lactobacillus and Bifidobacterium have been successfully used to promote growth and prevent diseases. Previous reports have demonstrated that Bacillus subtilis (B. subtilis) was a potential probiotic for animals. In this research, 180 B. subtilis were isolated from the soil, identified, and investigated in vitro. Furthermore, five B. subtilis were selected and mixed to investigate their effect on growth performance, immune response, intestine microbiota, and disease resistance in rabbits. Rabbits with a diet of 106 CFU g−1 mixed B. subtilis exhibited the best growth performance and higher serum IgG and IgA than controls (P < 0.05). Moreover, dairy with B. subtilis can promote the balance of intestinal flora. The major proinflammatory factor and β-defensin were upregulated compared with the controls. After 7 weeks of feeding, the survival rate of the rabbits fed with B. subtilis was significantly higher than those in the control groups postinfected with Escherichia coli. At the same time, this study detected higher expression of β-defensin and reduced bacteria contents of the heart and cecal contents from the diet mixed with B. subtilis compared with the control groups. In conclusion, dietary supplementation with B. subtilis for rabbits could improve growth performance, intestinal homeostasis, and immune organ index and enhance innate immune response as well as disease resistance. These findings showed that the induction of β-defensin by B. subtilis might be an interesting new therapeutic strategy to strengthen innate defense mechanisms.