High-resolution elasticity imaging for tissue engineering
High-resolution elasticity imaging for tissue engineering
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DOI：
10.1109/58.852079
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发表时间：
2000-07-01
影响因子：
3.6
通讯作者：
O'Donnell, M
中科院分区：
文献类型：
--
作者：
Cohn, NA;Kim, BS;O'Donnell, M
弹性显微镜提供组织弹性的高分辨率图像。有了这种仪器，有可能在组织工程中监测细胞生长和组织发育。为了验证这一假设，弹性显微照片中获得的两个模型系统通常用于组织工程。在第一个实验中，组织工程平滑肌样本的应变图像清楚地从其支持基质中识别出了数百微米厚的细胞层。由于一维力学模型适用于该系统，因此仅应变图像就足以对弹性特性进行成像。相反，第二个系统进行了研究，其中一个简单的一维力学模型是不够的。用弹性显微镜对包埋在均匀凝胶中的未培养胶原微球进行成像。单纯的应变图像不能清楚地描述硬球形细胞载体的弹性性质，然而，重建的弹性图像可以区分硬包含体和背景凝胶。这些结果有力地表明，弹性显微镜可能是一个有价值的工具，组织工程和其他应用程序需要在高空间分辨率（75 μ m或更小）的软组织的弹性特性。
An elasticity microscope provides high resolution images of tissue elasticity. With this instrument, it may be possible to monitor cell growth and tissue development in tissue engineering. To test this hypothesis, elasticity micrographs were obtained in two model systems commonly used for tissue engineering. In the first, strain images of a tissue-engineered smooth muscle sample clearly identified a several hundred micron thick cell layer from its supporting matrix. Because a one-dimensional mechanical model was appropriate for this system, strain images alone were sufficient to image the elastic properties. In contrast, a second system was investigated in which a simple one-dimensional mechanical model was inadequate. Uncultured collagen microspheres embedded in an otherwise homogeneous gel were imaged with the elasticity microscope. Strain images alone did not clearly depict the elastic properties of the hard spherical cell carriers, However, reconstructed elasticity images could differentiate the hard inclusion from the background gel. These results strongly suggest that the elasticity microscope may be a valuable tool for tissue engineering and other applications requiring the elastic properties of soft tissue at high spatial resolution (75 mu m or less).