Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses
Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses
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DOI：
10.1039/b808366e
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Grigoropoulos, Costas P.
中科院分区：
文献类型：
--
作者：
Kim, Moosung;Hwang, David J.;Grigoropoulos, Costas P.
我们展示了在熔融石英内部集成三维微通道和光波导结构的制造，用于单细胞的询问和处理。微通道是通过扫描飞秒激光脉冲（523 nm）和随后的选择性湿法刻蚀工艺制造的。通过扫描玻璃样品内的激光脉冲序列，光波导另外与制造的微通道集成。通过两种光学方案检测制造的微通道内稀释的人血中的单个红细胞（RBC）。第一个涉及传感波导传递的He-Ne激光（632.8 nm）的强度变化引起的折射率差的细胞在通道中流动。另一种方法是通过检测由光波导传递的Ar激光（488 nm）激发的来自染色RBC的荧光发射。通过将流速增加到0.5 μ l/min（-1），对所提出的装置进行了测试，以每秒检测23个荧光颗粒。光学细胞检测实验支持新一代基于玻璃的光流体生物芯片设备在各种单细胞处理过程中的潜在实施，包括基于激光的细胞处理和传感。
We demonstrate the fabrication of integrated three-dimensional microchannel and optical waveguide structures inside fused silica for the interrogation and processing of single cells. The microchannels are fabricated by scanning femtosecond laser pulses (523 nm) and subsequent selective wet etching process. Optical waveguides are additionally integrated with the fabricated microchannels by scanning the laser pulse train inside the glass specimen. Single red blood cells (RBC) in diluted human blood inside of the manufactured microchannel were detected by two optical schemes. The first involved sensing the intensity change of waveguide-delivered He-Ne laser light (632.8 nm) induced by the refractive index difference of a cell flowing in the channel. The other approach was via detection of fluorescence emission from dyed RBC excited by Ar laser light (488 nm) delivered by the optical waveguide. The proposed device was tested to detect 23 fluorescent particles per second by increasing the flow rate up to 0.5 mu l min(-1). The optical cell detection experiments support potential implementation of a new generation of glass-based optofluidic biochip devices in various single cell treatment processes including laser based cell processing and sensing.