Glycyrrhizic acid ameliorates sodium nitrite-induced lung and salivary gland toxicity: Impact on oxidative stress, inflammation and fibrosis
Glycyrrhizic acid ameliorates sodium nitrite-induced lung and salivary gland toxicity: Impact on oxidative stress, inflammation and fibrosis
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DOI：
10.1177/0960327120964555
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发表时间：
2020-10-08
影响因子：
2.8
通讯作者：
Elsherbiny, Nehal M.
中科院分区：
文献类型：
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作者：
Elsherbini, Amira M.;Maysarah, Nadia M.;Elsherbiny, Nehal M.
尽管亚硝酸钠（SN）作为食品添加剂被广泛应用，但其在高剂量或慢性暴露时对人体各器官表现出相当大的副作用。本研究的目的是测试甘草酸（GA）是否可以改善SN引起的肺和下颌下腺（SMG）毒性。将30只成年雄性白化病大鼠随机分为3组。第1组为对照组。在第2组中，大鼠经口给予80 mg/kg SN，或在第3组中，在SN之前给予（15 mg/kg）GA。肺和SMG组织用于氧化应激评估、组织病理学变化、纤维化（MTC、TGF-β和α-SMA）和炎症（TNF-α、IL-1 β和CD-68）的检查。同时给予GA通过降低MDA水平恢复抗氧化防御机制，改善了SN诱导的肺和唾液毒性。GA减少了纤维化TGF-β和α-SMA和胶原沉积的关键调节因子。除了减少炎性细胞因子（TNF-α，IL-1 β）和巨噬细胞的募集外，GA还改善了肺和唾液的形态学变化。本研究提出GA是一种有前途的天然草药，具有抗氧化、抗炎和抗纤维化作用，可对抗SN诱导的肺和唾液毒性。
Despite wide application of sodium nitrite (SN) as food additive, it exhibits considerable side effects on various body organs at high dose or chronic exposure. The aim of this study was to test whether Glycyrrhizic acid (GA) could ameliorate SN-induced toxicity in lung and submandibular salivary gland (SMG). A sample size of 30 adult male albino rats was randomly allocated into 3 groups. Group 1 served as control group. Rats were treated orally with 80 mg/kg of SN in group 2 or SN preceded by (15 mg/kg) GA in group 3. Lung & SMG tissues were used for oxidative stress assessment, examination of histopathological changes, fibrosis (MTC, TGF-beta and alpha-SMA) and inflammation (TNF-alpha, IL-1 beta and CD-68). Concurrent administration of GA ameliorated pulmonary and salivary SN-induced toxicity via restoring the antioxidant defense mechanisms with reduction of MDA levels. GA reduced the key regulators of fibrosis TGF-beta and alpha-SMA and collagen deposition. In addition to reduction of inflammatory cytokine (TNF-alpha, IL-1 beta) and macrophages recruitments, GA amended both pulmonary and salivary morphological changes. The present study proposed GA as a promising natural herb with antioxidant, anti-inflammatory and antifibrotic effects against pulmonary and salivary SN-induced toxicity.