Synthesis and continuous catalytic application of alkaline protease nanoflowers-PVA composite hydrogel
Synthesis and continuous catalytic application of alkaline protease nanoflowers-PVA composite hydrogel
复制标题
碱性蛋白酶纳米花-PVA复合水凝胶的合成及连续催化应用
DOI：
10.1016/j.catcom.2018.07.015
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发表时间：
2018
影响因子：
3.7
通讯作者：
Wang Yi
中科院分区：
文献类型：
--
作者：
Zhang Haiyang;Fei Xu;Tian Jing;Li Yao;Zhi Hui;Wang Kang;Xu Longquan;Wang Yi
以碱性蛋白酶-Cu 3（PO 4）2·3 H2O纳米花和聚乙烯醇（PVA）水凝胶为原料，采用冻融法制备了碱性蛋白酶纳米花-PVA复合水凝胶（NPCH）。在连续催化应用过程中，PVA水凝胶网络保护碱性蛋白酶-Cu 3（PO 4）2·3 H2O纳米花免受损伤，并有助于保持高水平的酶活性。固定化酶的酶活分别比游离地衣芽孢杆菌碱性蛋白酶提高了1027%和605%。当用于循环催化时，NPCH表现出比纳米花更好的可重复使用性，并且易于与产物分离。
This paper reports a facile approach for the synthesis of alkaline protease nanoflowers–poly(vinyl alcohol) (PVA) composite hydrogel (NPCH) from alkaline protease–Cu3(PO4)2·3H2O nanoflowers and PVA hydrogel through freezing–thawing. During continuous catalytic application, the PVA hydrogel network protected alkaline protease–Cu3(PO4)2·3H2O nanoflowers from damage and helped maintain enzymatic activity at high levels. The enzyme that had been immobilized in nanoflowers and NPCH demonstrated 1027% and 605% higher activity than the free alkaline protease derived fromBacillus licheniformis. When used in cyclic catalysis, NPCH exhibited better reusability than nanoflowers and was easily separated from the product.