Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level
Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level
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转录水平调控NIH3T3细胞周期的信号通路途径和方法分析
DOI：
10.1186/s12860-015-0071-7
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发表时间：
2015-10-28
期刊：
影响因子：
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通讯作者：
Xu, Cunshuan
中科院分区：
文献类型：
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作者：
Chang, Cuifang;Niu, Zhipeng;Xu, Cunshuan
背景：为了分析信号通路在转录水平调控NIH3T3细胞周期进程的途径和方法，我们对NIH3T3细胞周期进行了建模，发现NIH3T3细胞周期的G1期为同步化后5-15 h，S期为15-21 h，G2期为21-22 h，M期为22-25 h。 结果：小鼠基因组430 2.0芯片对模型的基因表达谱进行检测，结果显示NIH3T3细胞周期中64个细胞周期基因和960个与其他生理活动相关的基因表达发生显着变化。下一步，利用IPA软件分析这些基因的生理活性、细胞周期基因相关的信号转导活性及其在细胞周期进程中的调节作用，结果表明所报道的基因参与了17条信号通路对细胞周期进程的调节。新发现的基因如PKC、RAS、PP2A、NGR和PI3K等，属于乳腺癌信号通路中的癌症分子机制、细胞周期蛋白和细胞周期调节HER-2信号的功能范畴。这些新发现的基因可以促进DNA损伤修复和DNA复制进程，调节蛋白质代谢，并通过调节已报道的基因CCND1和C-FOS维持NIH3T3的细胞周期进程。结论：上述信号通路均与细胞周期网络相互作用，表明NIH3T3细胞周期受到多种信号通路的调控。
Background: To analyze the ways and methods of signaling pathways in regulating cell cycle progression of NIH3T3 at transcriptional level, we modeled cell cycle of NIH3T3 and found that G1 phase of NIH3T3 cell cycle was at 5-15 h after synchronization, S phase at 15-21 h, G2 phase at 21-22 h, M phase at 22-25 h.Results: Mouse Genome 430 2.0 microarray was used to detect the gene expression profiles of the model, and results showed remarkable changes in the expressions of 64 cell cycle genes and 960 genes associated with other physiological activity during the cell cycle of NIH3T3. For the next step, IPA software was used to analyze the physiological activities, cell cycle genes-associated signal transduction activities and their regulatory roles of these genes in cell cycle progression, and our results indicated that the reported genes were involved in 17 signaling pathways in the regulation of cell cycle progression. Newfound genes such as PKC, RAS, PP2A, NGR and PI3K etc. belong to the functional category of molecular mechanism of cancer, cyclins and cell cycle regulation HER-2 signaling in breast cancer signaling pathways. These newfound genes could promote DNA damage repairment and DNA replication progress, regulate the metabolism of protein, and maintain the cell cycle progression of NIH3T3 modulating the reported genes CCND1 and C-FOS.Conclusion: All of the aforementioned signaling pathways interacted with the cell cycle network, indicating that NIH3T3 cell cycle was regulated by a number of signaling pathways.