Inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium wilt disease
Inducing the rhizosphere microbiome by biofertilizer application to suppress banana Fusarium wilt disease
复制标题
施用生物肥料诱导根际微生物群抑制香蕉枯萎病
DOI：
10.1016/j.soilbio.2016.10.008
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发表时间：
2017-01-01
影响因子：
9.7
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
--
作者：
Fu, Lin;Penton, C. Ryan;Shen, Qirong
世界范围内，香蕉生产受到枯萎病的严重阻碍，枯萎病是一种由土传真菌尖孢镰刀菌引起的毁灭性疾病。sp. cubense（Foc.）由于没有广泛采用的有效控制或预防方法，一种新的Foc变异体，热带小种4（Foc TR 4）的出现，导致了卡文迪什产区种植园的广泛破坏。近年来，在新垦地连续施用生物肥料（BIO）防治香蕉枯萎病取得了良好的效果。在这项研究中，我们研究的时间效应的BIO与堆肥应用在新转换的香蕉田的根际细菌和真菌群落的组成和丰度和生存的生物控制接种剂，解淀粉芽孢杆菌NJN-6。我们的研究结果表明，生物改良的根际土壤增加了细菌的丰度，而减少真菌的丰度。这对应于BIO修正案中更高的细菌丰富度和多样性，而真菌群落没有观察到趋势。堆肥和生物有机肥处理对稻田土壤细菌和真菌群落组成的影响最大，而处理时间对稻田土壤细菌和真菌群落组成的影响不显著。还鉴定了其他参与疾病抑制的潜在分类群，例如鞘氨醇菌属、双孢菌属和隐球菌属的丰度增加，以及镰刀菌属、罗尔斯通氏菌属和伯克霍尔德氏菌属的丰度降低。总体而言，F.尖孢霉的生长和生物防治剂NJN-6的丰度在三年内缺乏变异性，与真菌和细菌组成和丰度的改变相结合，有助于疾病抑制，表明BIO作为疾病抑制的修正物的可持续性。(C)2016爱思唯尔有限公司版权所有
Worldwide, banana production is severely hindered by Fusarium wilt, a devastating disease caused by the soil-borne fungus Fusarium oxysporum f. sp. cubense (Foc). With no widely adopted efficient method of control or prevention, the emergence of a new Foc variant, tropical race 4 (Foc TR4), has led to the widespread destruction of plantations in Cavendish-producing areas. Recently, banana Fusarium wilt has been controlled by the consecutive application of biofertilizer (BIO) in newly reclaimed fields. In this study we examine the temporal effects of BIO versus compost application in newly converted banana fields on the composition and abundance of the rhizosphere bacterial and fungal communities and the survival of the biocontrol inoculant, Bacillus amyloliquefaciens NJN-6. Our findings show that BIO-amended rhizosphere soils increased the abundance of bacteria while decreasing fungal abundance. This corresponded to higher bacterial richness and diversity in the BIO amendment, while no trends were observed with the fungal community. Rhizosphere soil bacterial and fungal community composition were significantly different between BIO and compost amendment and treatment, not time, exhibited the largest impact. Other potential taxa involved in disease suppression were also identified, such as increased abundances of Sphingobium, Dyadobacter, and Cryptococcus and lower abundances of Fusarium, Ralstonia, and Burkholderia. Overall, decreased abundances of F. oxysporum and a lack of variability in the abundance of the biocontrol agent NJN-6 over three years contributed to disease suppression, in combination with alterations in fungal and bacterial composition and abundance, pointing to the sustainability of BIO as an amendment for disease suppression. (C) 2016 Elsevier Ltd. All rights reserved.