GIP, a G-protein-coupled receptor interacting protein
GIP, a G-protein-coupled receptor interacting protein
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DOI：
10.1016/s0167-0115(02)00201-x
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发表时间：
2002-11-15
影响因子：
--
通讯作者：
Birnbaumer, M
中科院分区：
文献类型：
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作者：
Innamorati, G;Whang, MI;Birnbaumer, M
在筛选与V2加压素受体（V2 R）的第二胞内环相互作用的伴侣时，克隆了一种新的蛋白质。该蛋白质被命名为GIP，与G蛋白偶联受体相互作用蛋白（G-protein-coupled receptor Interacting Protein）相同;相应的基因位于第17号染色体上，其中三个外显子编码一个379个氨基酸的蛋白质。结果表明，UP蛋白至少部分定位于细胞膜上，可能是以三聚体的形式存在，表明UP是一种跨膜蛋白，其大部分分子位于细胞内。序列同源性分析表明，GIP胞浆结构域折叠为胶原样螺旋结构，随后是球状结构域，球状结构域与V2 R的相互作用通过pull-down实验得到证实，表明球状结构域也可以与胞浆蛋白相互作用。(C)2002 Elsevier Science B. V.保留所有权利。
A novel protein was cloned while screening for partners interacting with the second intracellular loop of the V2 vasopressin receptor (V2R). The protein was named GIP as in G-protein-coupled receptor Interacting Protein; the corresponding gene was located on the 17th chromosome where three exons encode for a 379-amino-acid protein.GIP subcellular localization was studied by immunocytochemistry and also using a biotinylating agent. The protein was found to be localized, at least in part, on the plasma membrane, probably in the form of a trimer.The results indicated that UP is a transmembrane protein and the most part of the molecule is intracellular. Sequence homology inferred that GIP cytosolic domain is folded as a collagen-like helix followed by a globular domain.The interaction of the globular domain with the V2R was confirmed by pull-down experiments indicating that this structural motif can also interact with cytosolic proteins. (C) 2002 Elsevier Science B.V. All rights reserved.