In vitro small intestinal epithelial cell growth on a nanocomposite polycaprolactone scaffold
In vitro small intestinal epithelial cell growth on a nanocomposite polycaprolactone scaffold
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DOI：
10.1042/ba20090214
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Seifalian, Alexander M.
中科院分区：
文献类型：
--
作者：
Gupta, Ashish;Vara, Dina S.;Seifalian, Alexander M.
尽管全世界都在努力开发短肠综合征的功能性替代品，但小肠的组织工程仍处于实验阶段。大多数已发表的研究报告主要使用PLLA（聚-L-丙交酯酸）/PGA（聚乙醇酸）共聚物作为支架材料，并且研究受到体内实验的限制。这种缺乏进展激发了一个新的视角，并激发了组织工程这一领域的进一步研究和发展。在本文中，我们利用一个相对较新的纳米复合材料的POSS（多面体低聚倍半硅氧烷）和PCL [聚（己内酯脲）氨基甲酸酯]作为一种材料，开发多孔支架使用溶剂浇铸/颗粒沥滤技术，以制造不同的孔径和孔隙率的多孔支架。使用扫描电子显微镜和微计算机断层扫描对支架的孔形态和孔隙率进行表征。然后将大鼠肠上皮细胞接种到聚合物支架上，用于细胞相容性和增殖的体外研究，这通过接种后21天进行的Alcohol Blue（TM）和乳酸脱氢酶测定来评估。所获得的结果表明，POSS-PCL纳米复合材料作为大孔支架产生，其孔隙率在40-80%范围内，孔径在150-250 μ m范围内。该支架显示支持上皮细胞增殖和生长。总之，作为研究小肠组织工程的进一步步骤，在这项研究中采用的纳米复合材料可能被证明是一个有用的替代聚（乳酸-羟基乙酸）在未来。
Tissue engineering of the small intestine remains experimental despite worldwide attempts to develop a functional substitute for short bowel syndrome. Most published studies have reported predominant use of PLLA (poly-L-lactide acid)/PGA (polyglycolic acid) copolymer as the scaffold material, and studies have been limited by in vivo experiments. This lack of progress has inspired a fresh perspective and provoked further investigation and development in this field of tissue engineering. In the present paper, we exploit a relatively new nanocomposite of POSS (polyhedral oligomeric silsesquioxane) and PCL [poly(caprolactoneurea)urethane] as a material to develop porous scaffolds using a solvent casting/particulate leaching technique to fabricate porous scaffolds in different pore sizes and porosities. Scaffolds were characterized for pore morphology and porosity using scanning electron microscopy and micro-computed tomography. Rat intestinal epithelial cells were then seeded on to the polymer scaffolds for an in vitro study of cell compatibility and proliferation, which was assessed by Alamar Blue (TM) and lactate dehydrogenase assays performed for 21 days post-seeding. The results obtained demonstrate that POSS-PCL nanocomposite was produced as a macroporous scaffold with porosity over the range of 40-80% and pore size over the range of 150-250 mu m. This scaffold was shown to support epithelial cell proliferation and growth. In conclusion, as a further step in investigating small intestinal tissue engineering, the nanocomposite employed in this study may prove to be a useful alternative to poly(lactic-co-glycolic acid) in the future.