Acute orexin effects on insulin secretion in the rat: in vivo and in vitro studies.
Acute orexin effects on insulin secretion in the rat: in vivo and in vitro studies.
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DOI：
10.1016/s0024-3205(99)00611-6
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发表时间：
1999-12
期刊：
影响因子：
6.1
通讯作者：
K. Nowak;P. Maćkowiak;M. Świtońska;M. Fabiś;L. Malendowicz
中科院分区：
文献类型：
--
作者：
K. Nowak;P. Maćkowiak;M. Świtońska;M. Fabiś;L. Malendowicz
食欲素-A和食欲素-B是新近发现的下丘脑促食欲神经肽家族的成员。很少有数据表明食欲素参与调节垂体激素的分泌和能量稳态的控制。目前的研究旨在解释食欲素是否影响大鼠的血胰岛素浓度和胰岛素分泌。为了检查这种可能性，成年雌性大鼠皮下注射不同剂量（1或2 nmol）的食欲素-A或食欲素-B。食素-A的推注给药导致血液胰岛素（最多120分钟）和葡萄糖（注射后60分钟）浓度增加。另一方面，较高剂量的食欲素-B仅在实验的60分钟时对胰岛素分泌产生影响，并且两种剂量都不改变血糖水平。在大鼠胰腺制备的体外灌注系统中，只有食欲素A刺激胰岛素分泌，而食欲素B的效果较差。结果表明，食欲素属于一组影响胰岛素分泌的神经肽，直接作用于胰腺。食欲素对胰岛素分泌的直接的、至少部分的作用可能与这种肽对代谢的调节有关。
Orexin-A and orexin-B are members of a family of newly described orexigenic hypothalamic neuropeptides. Scanty data are available suggesting the involvement of orexins in regulation of the secretion of pituitary hormones and in control of energy homeostasis. Present studies aimed to explain whether orexins affect blood insulin concentration and insulin secretion in the rat. To check this possibility, adult female rats were subcutaneously injected with different doses (1 or 2 nmol) of orexin-A or orexin-B. A bolus administration of orexin-A resulted in an increase in blood insulin (up to min 120) and glucose (60 min after injection) concentration. The higher dose of orexin-B, on the other hand, exerted effect on insulin secretion only at min 60 of experiment and neither doses changed blood glucose level. Only orexin-A stimulated insulin secretion in an in vitro perfusion system of the rat pancreas preparation, while orexin-B was less effective. The results demonstrate that orexins belong to a group of neuropeptides influencing insulin secretion and acting directly on the pancreas. Direct, at least partial, effect of orexin on insulin secretion may be connected with the regulation of metabolism by this peptide.