Manganese Treatment Alleviates Zinc Deficiency Symptoms in Arabidopsis Seedlings
Manganese Treatment Alleviates Zinc Deficiency Symptoms in Arabidopsis Seedlings
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DOI：
10.1093/pcp/pcaa094
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发表时间：
2020-10-01
影响因子：
4.9
通讯作者：
Fukao, Yoichiro
中科院分区：
文献类型：
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作者：
Nakayama, Sayuri;Sugano, Shigeo S.;Fukao, Yoichiro
矿物质缺乏引起的植物表型因生长条件而异。我们最近报道，拟南芥的生长在基于 MGRL 的缺锌培养基上受到严重抑制，但在基于 Murashige-Skoog 的缺锌培养基上则不受抑制。在这里，我们探讨了在不同培养基上生长的拟南芥表型差异的根本原因。在短期生长期间（10或14天）添加额外的锰（Mn）可以挽救因缺锌而降低的根系生长和叶绿素含量。然而，这种处理并不影响长期生长（38天）后的生长恢复。为了研究植物从缺锌中恢复的原因，我们对在 Zn 基础培养基和含有/不含有额外 Mn 的贫锌培养基上生长的根进行了 RNA-seq 分析。 RNA-seq数据的主成分分析表明，在Zn基础培养基上植物的基因表达模式与在含Mn的贫锌培养基上的相似，而在不含Mn的贫锌培养基上的基因表达模式与其他培养基不同。仅在不含锰的贫锌培养基上的植物中，几种转录因子和活性氧（ROS）相关基因的表达上调。与基因表达数据一致，在该培养基上生长的根部中ROS的积累高于在其他条件下生长的根部。这些结果表明，植物在不良生长条件（例如锌消耗）下积累活性氧并减少其生物量。综上所述，这项研究表明，在缺锌培养基中添加额外的锰会诱导 ROS 相关基因的转录变化，从而减轻因缺锌而造成的短期生长抑制。
Plant phenotypes caused by mineral deficiencies differ depending on growth conditions. We recently reported that the growth of Arabidopsis thaliana was severely inhibited on MGRL-based zinc (Zn)-deficient medium but not on Murashige-Skoog-based Zn-deficient medium. Here, we explored the underlying reason for the phenotypic differences in Arabidopsis grown on the different media. The root growth and chlorophyll contents reduced by Zn deficiency were rescued by the addition of extra manganese (Mn) during short-term growth (10 or 14 d). However, this treatment did not affect the growth recovery after longterm growth (38 d). To investigate the reason for plant recovery from Zn deficiency, we performed the RNA-seq analysis of the roots grown on the Zn-basal medium and the Zn-depleted medium with/without additional Mn. Principal component analysis of the RNA-seq data showed that the gene expression patterns of plants on the Zn-basal medium were similar to those on the Zn-depleted medium with Mn, whereas those on the Zn-depleted medium without Mn were different from the others. The expression of several transcription factors and reactive oxygen species (ROS)-related genes was upregulated in only plants on the Zn-depleted medium without Mn. Consistent with the gene expression data, ROS accumulation in the roots grown on this medium was higher than those grown in other conditions. These results suggest that plants accumulate ROS and reduce their biomass under undesirable growth conditions, such as Zn depletion. Taken together, this study shows that the addition of extra Mn to the Zn-depleted medium induces transcriptional changes in ROS-related genes, thereby alleviating short-term growth inhibition due to Zn deficiency.