Purification, Quantification, and Functional Analysis of Collectins
Purification, Quantification, and Functional Analysis of Collectins
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集合素的纯化、定量和功能分析
DOI：
10.1007/978-1-0716-0430-4_10
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Wakamiya Nobutaka
中科院分区：
文献类型：
--
作者：
Ohtani Katsuki;Wakamiya Nobutaka
通过使用甘露聚糖-琼脂糖和抗凝集素抗体柱纯化天然和重组凝集素。使用两种抗人甘露聚糖结合凝集素（MBL）抗体的夹心酶联免疫吸附测定（ELISA）能够阐明血液、血清和血浆中的聚集素浓度。通过在糖结合试验中测定抑制50%糖结合所需的糖浓度来证明凝集素糖特异性。生物学分析包括补体依赖性溶血试验和其他几种方法用于评价凝集素。
Native and recombinant collectins are purified by using mannan-agarose and an anti-collectin antibody column. The use of sandwich enzyme-linked immunosorbent assay (ELISA) with two antibodies against human mannan-binding lectin (MBL) enables elucidation of the collectin concentration in the blood, serum, and plasma. The collectin sugar specificity is demonstrated by determining the concentration of saccharide required to inhibit sugar binding by 50% in a saccharide-binding assay. Biological analyses including the complement-dependent hemolysis test and several other methods are used to evaluate collectin.