The star-type color pattern in Petunia hybrida 'Red star' flowers is induced by sequence-specific degradation of chalcone synthase RNA
The star-type color pattern in Petunia hybrida 'Red star' flowers is induced by sequence-specific degradation of chalcone synthase RNA
复制标题
DOI：
10.1093/pcp/pci192
复制
发表时间：
2005-11-01
影响因子：
4.9
通讯作者：
Kanazawa, A
中科院分区：
文献类型：
--
作者：
Koseki, M;Goto, K;Kanazawa, A
红星矮牵牛花是一种花呈星形红白双色图案的品种。我们分析了参与花青素生物合成的6个基因的mRNA水平。只有查尔酮合成酶（chalcone synthase， CHS） mRNA水平在未着色的花区受到抑制。检测了CHS在未着色部分的转录活性和短干扰RNA的积累。病毒感染阻断了chs沉默区的产生。这些结果表明，CHS RNA的序列特异性降解是红星花白色部分形成的主要原因。
Petunia hybrida 'Red Star' is a variety whose flowers exhibit a star-type red and white bicolor pattern. We analyzed the mRNA levels of six genes involved in anthocyanin biosynthesis. Only the level of chalcone synthase (CHS) mRNA was depressed in the unpigmented flower sectors. Both transcriptional activity and the accumulation of short interfering RNA of CHS in the unpigmented sectors were detected. Viral infection blocked the generation of CHS-silenced sectors. These results indicate that sequence-specific degradation of CHS RNA is the primary cause of the formation of white sectors in 'Red Star' flowers.