An Experimental Model of Urethral Stricture in Rabbits Using Holmium Laser under Urethroscopic Direct Visualization
An Experimental Model of Urethral Stricture in Rabbits Using Holmium Laser under Urethroscopic Direct Visualization
复制标题
尿道镜直接可视化钬激光兔尿道狭窄实验模型
DOI：
10.1159/000355353
复制
发表时间：
2014-01-01
影响因子：
1.6
通讯作者：
Zeng, Xiao-Yong
中科院分区：
文献类型：
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作者：
Hu, Wei-Feng;Li, Cui-Ling;Zeng, Xiao-Yong
目的：建立尿道镜直视下钬激光治疗兔尿道狭窄的动物模型。方法：16只成年雄性新西兰兔随机分为对照组和实验组。所有家兔在肌肉注射麻醉剂后进行逆行尿道造影和经尿道内窥镜检查。我们使用钬激光：YAG激光在直接可视化下损伤实验组所有家兔的远端尿道。术后30天，所有动物均接受逆行尿道造影和尿道镜检查。测量离体尿道的流速以评估尿道狭窄的形成。结果：实验组1只（12.5%）兔于术后4d死于感染。术后30天，逆行尿道造影和尿道镜检查显示实验组所有7只存活家兔（87.5%）均出现狭窄。实验组离体尿道的平均流速明显低于对照组。结论：在尿道镜直视下应用钬激光可成功建立兔尿道狭窄模型，为研究尿道狭窄的发病机制和分子生物学提供了理想的对象。(C)2014 S. Karger AG，巴塞尔
Objective: To establish an experimental rabbit model of urethral stricture using holmium laser under direct urethroscopic visualization. Methods: Sixteen adult male New Zealand rabbits were divided into equally-sized control and experimental groups. All rabbits underwent retrograde urethrography and transurethral endoscopy with a 7.5 F urethroscope after intramuscular anesthetic injection. We used a holmium:YAG laser to injure the distal urethra in all rabbits in the experimental group under direct visualization. Thirty days after surgery, all animals were evaluated with retrograde urethrography and urethroscopy. The flow rate of the isolated urethras was measured to evaluate urethral stricture formation. Results: One rabbit in the experimental group (12.5%) died of infection 4 days after surgery. Thirty days after surgery, retrograde urethrography and urethroscopy revealed strictures in all seven surviving rabbits (87.5%) in the experimental group. The mean flow rate of the isolated urethras was significantly lower in the experimental group than in the control group. Conclusion: A rabbit model of urethral stricture can be successfully established using holmium laser under direct urethroscopic visualization, providing an ideal object for research concerning the pathogenesis and molecular biology of urethral strictures. (C) 2014 S. Karger AG, Basel