Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits-猫眼课题宝

DOI：

10.1101/2023.07.17.549299

发表时间：

2023-08

期刊：

bioRxiv

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

C. Bian;Gozde S. Demirer;M. Oz;Y. Cai;S. Witham;GA Mason;R. Shen;A. Gaudinier;SM Brady;NJ Patron

氮是植物所有生命过程中必不可少的元素。因此，植物根通过执行信号传导和转录级联反应动态地响应地下的氮可用性，从而导致改变的植物生长，优化养分吸收。NIN样蛋白7（NLP 7）转录因子感知氮，并且沿着与其密切相关的parasitic NLP 6部分协调这些转录应答。在这里，我们剖析了拟南芥氮转录网络的一个子回路，包括生长素反应因子18（ARF 18）、ARF 9、脱水反应元件结合蛋白质26（DREB 26）、含有蛋白质32（ANAC 032）的NAC结构域、NLP 6和NLP 7转录因子及其对硝酸还原酶1（NIR 1）的调节。这些因子的顺式调控结构被定义为直接转录调控，导致具有多个多节点前馈环的网络。该网络的保守性和发散性在番茄中也进行了类似的评估。所得到的模型进行验证，通过测量的转录输出的网络与合成的硝酸盐调节启动子在不同浓度的可用的硝酸盐揭示重新布线的氮调节不同的植物谱系。氮是植物生长和产量的重要营养元素。虽然外源氮促进了现代农业的发展，但过量施用含氮肥料会产生严重的生态和环境后果。在这里，我们专注于一个调控电路的关键NIN样蛋白7转录因子的上游和它的保守性和分歧之间的拟南芥和番茄。这些物种之间存在基因成员、阻遏物、前馈和多节点环的差异。由此产生的网络模型为有针对性的工程提供了一个框架，以提高植物氮素利用效率。

Nitrogen is an essential element for all life processes in plants. As such, plant roots dynamically respond to nitrogen availability below-ground by executing a signaling and transcriptional cascade resulting in altered plant growth, optimized for nutrient uptake. The NIN-LIKE PROTEIN 7 (NLP7) transcription factor senses nitrogen and along with its closely related paralog NLP6, partially coordinates these transcriptional responses. Here, we dissect a sub-circuit of the Arabidopsis nitrogen transcriptional network comprising AUXIN RESPONSE FACTOR 18 (ARF18), ARF9, DEHYDRATION RESPONSE ELEMENT BINDING-PROTEIN 26 (DREB26), A NAC-DOMAIN CONTAINING PROTEIN 32 (ANAC032), NLP6 and NLP7 transcription factors and their regulation of NITRATE REDUCTASE 1 (NIR1). The cis-regulatory architecture of these factors are defined as is direct transcriptional regulation resulting in a network with multiple multi-node feedforward loops. Conservation and divergence of this network is similarly assessed in Solanum lycopersicum. The resulting models are validated by measuring the transcriptional output of the network with the synthetic NITRATE-REGULATED PROMOTER in varying concentrations of available nitrate revealing rewiring of nitrogen regulation across distinct plant lineages. Significance Statement Nitrogen is a critical nutrient for plant growth and yield. While external N has facilitated modern agriculture, over-application of N-containing fertilizers has drastic ecological and environmental consequences. Here, we focus on a regulatory circuit acting upstream of the critical NIN-LIKE PROTEIN7 transcription factor and its conservation and divergence between Arabidopsis and tomato. Differences in gene membership, repressors, feedforward, and multinode loops exist between these species. The resulting network models provide a framework for targeted engineering to increase plant nitrogen use efficiency.

Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits

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