Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm
Near-field fluorescence imaging of single molecules with a resolution in the range of 10 nm
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DOI：
10.1046/j.1365-2818.2001.00815.x
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发表时间：
2001-05-01
影响因子：
2
通讯作者：
Saiki, T
中科院分区：
文献类型：
--
作者：
Hosaka, N;Saiki, T
我们用近场扫描光学显微镜(NSOM)演示了单分子的荧光成像，使用了光圈探头的照明收集操作模式。获得了单个染料分子的荧光图像，其空间分辨率为15 nm，小于所用探头的孔径(20 Nm)。这种超分辨率可以归因于从分子到探针涂层金属的非辐射能量传递，因为在常规NSOM的情况下，由于光穿透到金属中，获得的分辨率被限制在30-50 nm。
We demonstrate fluorescence imaging of single molecules, by near-field scanning optical microscopy (NSOM), using the illumination-collection mode of operation, with an aperture probe. Fluorescence images of single dye molecules were obtained with a spatial resolution of 15 nm, which is smaller than the diameter of the aperture (20 nm) of the probe employed. Such super-resolution may be attributable to non-radiative energy transfer from the molecules to the coated metal of the probe since the resolution obtained in the case of conventional NSOM is limited to 30-50 nm due to penetration of light into the metal.