Progenitor stem cell marker expression by pulmonary carcinomas
Progenitor stem cell marker expression by pulmonary carcinomas
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DOI：
10.1038/modpathol.2010.68
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发表时间：
2010-06-01
期刊：
影响因子：
7.5
通讯作者：
Downey, Robert J.
中科院分区：
文献类型：
--
作者：
Moreira, Andre L.;Gonen, Mithat;Downey, Robert J.
癌可能以再生障碍的形式出现，因此恶性细胞可能代表未能完全获得分化组织的特征。我们假设不同组织学类型的肺癌中祖细胞标记物的分布存在差异，分化差的肿瘤更有可能表达祖细胞标记物。该研究仅限于已切除的未经治疗的肺癌的石蜡包埋档案材料，包括腺癌、鳞状细胞癌、大细胞癌和小细胞癌。对切片进行假定的干细胞标记物（Musashi-1、Musashi-2、CD34、CD21、KIT、CD133、p63 和 OCT-4）染色。阳性被解读为孤立的、局灶性的或弥漫性的染色。在所有组织学类型的肺癌中均检测到干细胞标志物。组织学类型之间标记物的表达存在差异。小细胞癌大多数标记物呈弥漫性阳性；与其他组织学组中的局灶性或阴性染色相反。观察到 CD21 和 Musashi-1 之间呈负相关。在任何肿瘤类型中均未观察到 OCT-4 和 CD34 染色。基于标记表达的分层聚类将肿瘤分为两组，其中一组以 Musashi-1 和 KIT 高表达为标记，包含大部分低分化腺癌和小细胞癌。因此，干细胞标记物在肺癌中的表达对于不同的组织学类型和分化程度具有不同的模式。现代病理学(2010) 23, 889-895; doi:10.1038/modpathol.2010.68； 2010 年 3 月 19 日在线发布
Carcinomas may arise as a disorder of regeneration, so that a malignant cell may represent a failure to fully attain the characteristics of differentiated tissue. We hypothesized that there is a differential distribution of progenitor cell markers among different histological types of lung cancers, with poorly differentiated tumors being more likely to express progenitor stem cell markers. The study was limited to paraffin-embedded archival material of resected untreated pulmonary carcinomas, including adenocarcinoma, squamous cell carcinoma, large cell carcinoma, and small cell carcinoma. The sections were stained for putative stem cells markers (Musashi-1, Musashi-2, CD34, CD21, KIT, CD133, p63, and OCT-4). Positivity was read as isolated, focal, or diffuse staining. Stem cell markers were detected in all histological types of pulmonary carcinomas. There was a difference in the expression of markers among the histological types. Small cell carcinoma showed diffuse positivity for most of the markers; in contrast to focal or negative staining in other histological groups. An inverse relationship between CD21 and Musashi-1 was observed. No staining for OCT-4 and CD34 was seen in any of the tumor types. Hierarchical clustering based on marker expression separated tumors into two groups, with one group marked by high expression of Musashi-1 and KIT, contained most of the poorly differentiated adenocarcinomas and small cell carcinomas. Therefore, stem cell markers are expressed in lung cancers with different patterns seen for different histological types and degrees of differentiation. Modern Pathology (2010) 23, 889-895; doi:10.1038/modpathol.2010.68; published online 19 March 2010