A role for β-actin in RNA polymerase III transcription
A role for β-actin in RNA polymerase III transcription
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DOI：
10.1101/gad.1250804
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发表时间：
2004-12-15
影响因子：
10.5
通讯作者：
Hernandez, N
中科院分区：
文献类型：
--
作者：
Hu, P;Wu, S;Hernandez, N
当用重组因子和纯化的RNA聚合酶III（pol III）重建来自人U6 snRNA基因的转录时，pol III必须用CK 2处理才有活性。我们表明，高度纯化的聚合酶III含有相关的β-肌动蛋白，和β-肌动蛋白定位到一个活跃的U6启动子在体内。从用遗传毒性剂处理的IMR 90细胞中免疫沉淀的Pol III缺乏相关的β-肌动蛋白，并且在重构测定中无活性。用CK 2处理pol III并加入β-肌动蛋白后，转录恢复。这表明与pol III相关的β-肌动蛋白对于基础pol III转录是必需的。
When transcription from the human U6 snRNA gene is reconstituted with recombinant factors and purified RNA polymerase III (pol III), pol III must be treated with CK2 to be active. We show that highly purified pol III contains associated beta-actin, and beta-actin localizes to an active U6 promoter in vivo. Pol III immunoprecipitated from IMR90 cells treated with a genotoxic agent lacks associated beta-actin and is inactive in the reconstituted assay. Transcription is regained upon treatment of pol III with CK2 and addition of beta-actin. This suggests that beta-actin associated with pol III is essential for basal pol III transcription.