The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing
The Multiple Roles of Cohesin in Meiotic Chromosome Morphogenesis and Pairing
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DOI：
10.1091/mbc.e08-06-0637
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发表时间：
2009-02-01
影响因子：
3.3
通讯作者：
Amon, Angelika
中科院分区：
文献类型：
--
作者：
Brar, Gloria A.;Hochwagen, Andreas;Amon, Angelika
姐妹染色单体凝聚力由凝聚素复合物介导，在DNA复制过程中形成，对于染色体的准确分离至关重要。以往的研究表明，除了它们的凝聚功能，cohesins是必不可少的完成重组，配对，减数分裂染色体轴的形成，和组装的联会复合体（SC）。使用突变体的凝聚素亚基Rec8，其中磷酸化残基突变为丙氨酸，我们表明，凝聚素磷酸化不仅是重要的凝聚素去除，但凝聚素的减数分裂前期功能是不同的。我们发现配对和SC形成依赖于Rec8，但独立于姐妹染色单体的存在，因此姐妹染色单体的凝聚力。我们确定了REC8中的突变，这些突变差异地影响Rec8的凝聚力、配对、重组、染色体轴和SC组装功能。这些发现将Rec8定义为减数分裂染色体形态发生的关键决定因素和多个减数分裂事件的中心参与者。
Sister chromatid cohesion, mediated by cohesin complexes, is laid down during DNA replication and is essential for the accurate segregation of chromosomes. Previous studies indicated that, in addition to their cohesion function, cohesins are essential for completion of recombination, pairing, meiotic chromosome axis formation, and assembly of the synaptonemal complex (SC). Using mutants in the cohesin subunit Rec8, in which phosphorylated residues were mutated to alanines, we show that cohesin phosphorylation is not only important for cohesin removal, but that cohesin's meiotic prophase functions are distinct from each other. We find pairing and SC formation to be dependent on Rec8, but independent of the presence of a sister chromatid and hence sister chromatid cohesion. We identified mutations in REC8 that differentially affect Rec8's cohesion, pairing, recombination, chromosome axis and SC assembly function. These findings define Rec8 as a key determinant of meiotic chromosome morphogenesis and a central player in multiple meiotic events.