Genetic and biochemical characterization of CGB-1, an ambler class B carbapenem-hydrolyzing β-lactamase from Chryseobacterium gleum
Genetic and biochemical characterization of CGB-1, an ambler class B carbapenem-hydrolyzing β-lactamase from Chryseobacterium gleum
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DOI：
10.1128/aac.46.9.2791-2796.2002
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发表时间：
2002-09-01
影响因子：
4.9
通讯作者：
Nordmann, P
中科院分区：
文献类型：
--
作者：
Bellais, S;Naas, T;Nordmann, P
胶黄杆菌(以前包括在黄杆菌IIb种中)是一种革兰氏阴性需氧菌，是医院感染的来源之一。从能降低对超广谱头孢菌素和碳青霉烯类药物敏感性的参比菌株C.golum CIP 103039中克隆并在大肠杆菌中表达Ambler B类β-内酰胺酶基因。纯化的β-内酰胺酶CGB-1，等电点为8.6，测定相对分子质量约为26 kDa，包括水解性青霉素类、窄谱和广谱头孢菌素类以及碳青霉烯类。CGB-1是金属酶分子亚类B1中的一个新成员。该基因与吲哚金杆菌IND-1和脑膜败血杆菌BLAB的氨基酸同源性分别为83%和42%。因此，除了先前鉴定的克拉维酸抑制的Ambler A类超广谱β-内酰胺酶CGA-1外，胶囊藻还产生一种非常可能的染色体携带的B类β-内酰胺酶。
Chryseobacterium gleum (previously included in the Flavobacterium IIb species) is a gram-negative aerobe that is a source of nosocomial infections. An Ambler class B beta-lactamase gene was cloned and expressed in Escherichia coli from reference strain C. gleum CIP 103039 that had reduced susceptibility to expanded-spectrum cephalosporins and carbapenems. The purified beta-lactamase, CGB-1, with a pI value of 8.6 and a determined relative molecular mass of ca. 26 kDa, hydrolyzed penicillins; narrow- and expanded-spectrum cephalosporins; and carbapenems. CGB-1 was a novel member of the molecular subclass B1 of metallo-enzymes. It had 83 and 42% amino acid identity with IND-1 from Chryseobacterium indologenes and BlaB from C. meningosepticum, respectively. Thus, in addition to the previously characterized clavulanic acid-inhibited extended-spectrum beta-lactamase CGA-1 of Ambler class A, C. gleum produces a very likely chromosome-borne class B beta-lactamase.