The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer
The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer
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根除幽门螺杆菌对十二指肠溃疡患者胃肠道微生物群的影响
DOI：
10.15403/jgld.2014.1121.252.hpe
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发表时间：
2016-06-01
影响因子：
2.1
通讯作者：
Zhang, Guoxin
中科院分区：
文献类型：
--
作者：
Li, Lin;Zhou, Xiaoying;Zhang, Guoxin
背景：最近的研究表明，幽门螺杆菌（H. pylori）可能对胃肠道植物群产生影响，而且胃肠道细菌在十二指肠溃疡（DU）的发生发展中也有一定作用。目的：本研究旨在评价H.方法：2013年12月至2014年4月在江苏省盛泽医院进行病例对照研究。如果H.幽门螺杆菌检测呈阳性根除治疗停止后至少4周，再次进行胃镜检查，在相同位置再次采集活检标本。采集胃粘膜标本和粪便标本，提取细菌DNA，采用实时荧光定量聚合酶链反应（PCR）进行定量分析。胃窦乳酸菌群、纤细梭菌群、肠杆菌群增加，球状梭菌群减少。在语料库中，乳酸菌群中的细菌数量增加，球状梭菌亚群的表达显著下调。在粪便样品中，只有乳酸菌组的数量增加。结论：H。胃内pylori抑制乳酸菌群、纤细梭菌群和肠杆菌的定植。性别可能会影响DU患者中细菌的分布和/或繁殖。
Background: Recent reports have indicated that Helicobacter pylon (H. pylori) might have an effect on gastrointestinal flora; moreover, gastric commensual bacteria have been observed in the development of duodenal ulcer (DU). Aims: In our study, we aimed to evaluate the effect of H. pylon eradication on gastrointestinal flora in DU patients.Methods: A case-control study was performed at Jiangsu Shengze Hospital between December, 2013 and April, 2014. The patients received antibiotic eradication therapy if H. pylori testing was positive. At least four weeks after cessation of the eradication therapy, a repeat gastroscopy was performed to collect biopsies again in the same position. Gastric mucosa samples and feces specimens were collected to extract bacteria DNA and then to quantify by real-time polymerase chain reaction (PCR).Results: After the eradication of H. pylon, an increase of Lactobacillus group, Clostridium leptum subgroup, Enterobacteria and a decrease of Clostridium coccoides subgroup were found in the antrum. In the corpus, the number of bacteria in the Lactobacillus group was increased and the expression of Clostridium coccoides subgroup was significantly down-regulated. In the feces samples, only the number in the Lactobacillus group was increased. Moreover, the distribution was significantly different between female and the male patients.Conclusions: The presence of H. pylori in the stomach suppressed the colonization with Lactobacillus group, Clostridium leptum subgroup and Enterobacteria. Gender might affect the distribution and/or recolonization of the bacteria in DU patients.