ISOLATION AND COMPUTER-AIDED CHARACTERIZATION OF MMEI, A TYPE-II RESTRICTION ENDONUCLEASE FROM METHYLOPHILUS-METHYLOTROPHUS
ISOLATION AND COMPUTER-AIDED CHARACTERIZATION OF MMEI, A TYPE-II RESTRICTION ENDONUCLEASE FROM METHYLOPHILUS-METHYLOTROPHUS
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DOI：
10.1093/nar/14.13.5255
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发表时间：
1986-07-11
影响因子：
14.9
通讯作者：
BRAMMAR, WJ
中科院分区：
文献类型：
--
作者：
BOYD, AC;CHARLES, IG;BRAMMAR, WJ
II 型限制性内切核酸酶 MmeI 已从专性甲基营养菌 Mmethylophilusmethylotropicus 中纯化出来。该酶显示出具有非回文识别序列 5'-T C C Pu A C (N)20-3'、3'-A G G Py TG (N)18-5'，并在 3' 侧进行切割（如图所示），产生两个核苷酸的 3' 投影。使用两个新的计算机程序确定了识别序列； RECOG，根据从已知序列的 DNA 获得的限制性片段的模式预测识别序列；GELSIM，生成通过测序 DNA 分子的限制性消化的凝胶电泳获得的 DNA 带模式的图形模拟。图片
A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules. Images