The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.
The phosphocreatine shuttle of sea urchin sperm: flagellar creatine kinase resulted from a gene triplication.
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海胆精子的磷酸肌酸穿梭：鞭毛肌酸激酶由基因三倍体产生。
DOI：
10.1073/pnas.87.13.5203
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发表时间：
1990
影响因子：
11.1
通讯作者：
Shapiro,BM
中科院分区：
文献类型：
--
作者：
Wothe,DD;Charbonneau,H;Shapiro,BM
TCK，来自海胆Strongylocentrotus purpuratus精子鞭毛的肌酸激酶（ATP：肌酸N-磷酸转移酶），是一种Mr 145，000的轴丝蛋白，用于能量运输。其氨基酸序列通过溴化氰消化的片段分析和从两个海胆睾丸文库中克隆的cDNA测序获得。TCK含有三个完整但不相同的肌酸激酶片段，这些片段由非肌酸激酶样序列区域连接，两侧是独特的氨基和羧基末端。每个肌酸激酶片段与肌肉和大脑类型的脊椎动物肌酸激酶同源，并且所有三个重复序列都含有必需的活性位点半胱氨酸。重复序列之间的差异表明一个古老的基因三倍，在脊索动物棘皮动物分歧的时间。棘皮动物精子中有一种独特的肌酸激酶，卵子中有精氨酸激酶，体细胞中有两种磷酸原激酶，这可能代表了进化中一个保存下来的分支点，TCK可能是这一事件的遗迹。
TCK, the creatine kinase (ATP:creatine N-phosphotransferase) from sperm flagella of the sea urchin Strongylocentrotus purpuratus, is a Mr 145,000 axonemal protein that is employed in energy transport. Its amino acid sequence was obtained by analysis of fragments from cyanogen bromide digestion and by sequencing cDNA clones from two sea urchin testis libraries. TCK contains three complete but nonidentical creatine kinase segments joined by regions of sequence that are not creatine kinase-like and flanked by unique amino and carboxyl termini. Each creatine kinase segment is homologous to vertebrate creatine kinases of both muscle and brain types, and all three repeats contain the essential active-site cysteine. The sequence differences among repeats suggest an ancient gene triplication, around the time of the chordate-echinoderm divergence. The echinoderm, with a unique creatine kinase in sperm, arginine kinase in eggs, and both phosphagen kinases in somatic cells, may represent a preserved branch point in evolution, and TCK may be a relic of this event.