Characterization of peripheral hematopoietic stem cells and monocytes in Parkinson's disease
Characterization of peripheral hematopoietic stem cells and monocytes in Parkinson's disease
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DOI：
10.1002/mds.25300
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发表时间：
2013-03-01
影响因子：
8.6
通讯作者：
Biskup, Saskia
中科院分区：
文献类型：
--
作者：
Funk, Natalja;Wieghofer, Peter;Biskup, Saskia
背景新出现的证据强调了免疫系统在神经退行性疾病中的关键作用。本研究探讨了进行性神经变性对帕金森病患者外周血造血干细胞分化和发育的影响。研究方法：建立了帕金森病患者外周血造血干细胞集落形成细胞实验，并采用流式细胞术检测了帕金森病患者外周血单核细胞趋化因子受体的表达。结果我们证明，帕金森病患者和无症状的高危个体外周血中单核细胞前体细胞的百分比有强烈的上调。我们确定受体CCR2在帕金森病患者的经典单核细胞表面上经历强烈的上调。结论帕金森病患者脑内血细胞发育和进行性细胞死亡之间的关系应作为一种潜在的动态生物标志物和疾病进展的指标进行进一步研究。(c)2013年运动障碍协会
Background Emerging evidence has highlighted the pivotal role of the immune system in neurodegenerative diseases. This study investigated the impact of progressive neurodegeneration on the differentiation and development of hematopoietic stem cells in the peripheral blood of Parkinson's patients. Methods: A colony-forming cell assay was established to study hematopoietic stem cells from venous blood of Parkinson's patients, and flow cytometry was used to analyze the expression of chemokine receptors on monocytes. Results We demonstrate that there is strong upregulation in the percentage of monocyte precursors in the peripheral blood of Parkinson's patients and asymptomatic high-risk individuals. We identify the receptor CCR2 as undergoing strong upregulation on the surface of classical monocytes in Parkinson's patients. Conclusions The association between blood cell development and progressive cell death in the brain of Parkinson's patients should be further investigated as a potential dynamic biomarker and indicator of disease progression. (c) 2013 Movement Disorder Society