Interplay between septins and ubiquitin-mediated xenophagy during Shigella entrapment.
Interplay between septins and ubiquitin-mediated xenophagy during Shigella entrapment.
复制标题
志贺氏菌捕获过程中败血症与泛素介导的异体吞噬之间的相互作用。
DOI：
10.1080/27694127.2023.2213541
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Mostowy,Serge
中科院分区：
文献类型：
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作者：
Lobato-Márquez,Damián;Conesa,JoséJavier;López-Jiménez,AnaTeresa;Divine,MichaelE;Pruneda,JonathanN;Mostowy,Serge
Septins是一种细胞骨架蛋白，参与许多细胞过程，包括胞质分裂和形态发生。在福氏志贺氏菌感染的情况下，隔膜组装成笼状结构，捕获自噬靶标的胞液细菌。关于隔膜笼包埋和细菌自噬之间的相互作用，人们知之甚少。我们使用相关的光和低温软X射线层析成像(CRYO-SXT)管道来研究壳聚糖笼包埋志贺兰的近天然状态。Septin笼子可以被鉴定为X射线致密结构，表明它们含有与其自噬连接一致的宿主细胞蛋白质和脂类。志贺氏菌-Septin笼的AiryScan共聚焦显微镜显示，Septins和赖氨酸63(K63)连接的泛素链存在于不同的细菌微域中，这表明它们是单独招募的。最后，冷冻-SXT和活细胞成像显示，在志贺氏菌的自噬过程中，间隔蛋白和微管相关蛋白轻链3B(LC3B)阳性膜之间存在相互作用。总的来说，我们的数据为Septin-CagedShigellaas如何靶向自噬提供了一个新的模型。
Septins are cytoskeletal proteins implicated in numerous cellular processes including cytokinesis and morphogenesis. In the case of infection byShigella flexneri, septins assemble into cage-like structures that entrap cytosolic bacteria targeted by autophagy. The interplay between septin cage entrapment and bacterial autophagy is poorly understood. We used a correlative light and cryo-soft X-ray tomography (cryo-SXT) pipeline to study septin cage entrapment ofShigellain its near-native state. Septin cages could be identified as X-ray dense structures, indicating they contain host cell proteins and lipids consistent with their autophagy links. Airyscan confocal microscopy ofShigella-septin cages showed that septins and lysine 63 (K63)-linked ubiquitin chains are present in separate bacterial microdomains, suggesting they are recruited separately. Finally, Cryo-SXT and live-cell imaging revealed an interaction between septins and microtubule-associated protein light chain 3B (LC3B)-positive membranes during autophagy ofShigella. Collectively our data present a new model for how septin-cagedShigellaare targeted to autophagy.