Bombyx mori single repeat telomeric DNA sequence forms a G-quadruplex capped by base triads
Bombyx mori single repeat telomeric DNA sequence forms a G-quadruplex capped by base triads
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DOI：
10.1038/nsb0597-382
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发表时间：
1997-05-01
期刊：
影响因子：
--
通讯作者：
Patel, DJ
中科院分区：
文献类型：
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作者：
Kettani, A;Bouaziz, S;Patel, DJ
核磁共振-分子动力学相结合的方法已被用来确定家蚕端粒 d(TTAGG) 单重复序列的截短类似物在含 Na+ 阳离子水溶液中的溶液结构。双重对称四链 d(TAGG) 四链体包含两个相邻的 G(syn)。 G（同步）。 G（反）。 G（抗）G-四分体夹在新颖的（T.A）之间。具有独立链的三联体，在四链体周围具有平行和反平行邻居。 (T.A)。三联体代表了碱基三联体排列的第一个实验验证，它构成了最近提出的三联体-DNA模型中的关键假设，此外，(T.A)。通过氢键将 A 残基定位在 Watson-Crick T 的小沟中，生成三联体。碱基对，包括与最近在四膜虫自剪接 I 族核酶的 P4-P6 结构域结构中观察到的 A-A 平台相关的 T-A 平台。截短的家蚕四联体结构的新颖结构为额外碱基四联体和三联体的设计和潜在鉴定奠定了基础，这些四联体和三联体可以在染色体关联和遗传重组过程中参与多链DNA结构的配对比对。
A combined NMR-molecular dynamics approach has been applied to determine the solution structure of a truncated analogue of the Bombyx mori telomeric d(TTAGG) single repeat sequence in Na+ cation-containing aqueous solution. The two-fold symmetric four-stranded d(TAGG) quadruplex contains two adjacent G(syn). G(syn). G(anti). G(anti) G-tetrads sandwiched between novel (T . A). A triads with individual strands having both a parallel and antiparallel neighbour around the quadruplex. The (T . A). A triad represents the first experimental verification of a base triad alignment which constitutes a key postulate in the recently proposed model of triad-DNA, Further, the (T . A). A triad is generated by positioning an A residue through hydrogen bonding in the minor groove of a Watson-Crick T . A base pair and includes a T-A platform related to an A-A platform recently observed in the structure of the P4-P6 domain of the Tetrahymena self splicing group I ribozyme. The novel architecture of the truncated Bombyx mori quadruplex structure sets the stage for the design and potential identification of additional base tetrads and triads that could participate in pairing alignments of multi-stranded DNA structures during chromosome association and genetic recombination.