CLONING, EXPRESSION, AND DNA-SEQUENCE ANALYSIS OF GENES ENCODING NONTYPABLE HAEMOPHILUS-INFLUENZAE HIGH-MOLECULAR-WEIGHT SURFACE-EXPOSED PROTEINS RELATED TO FILAMENTOUS HEMAGGLUTININ OF BORDETELLA-PERTUSSIS
CLONING, EXPRESSION, AND DNA-SEQUENCE ANALYSIS OF GENES ENCODING NONTYPABLE HAEMOPHILUS-INFLUENZAE HIGH-MOLECULAR-WEIGHT SURFACE-EXPOSED PROTEINS RELATED TO FILAMENTOUS HEMAGGLUTININ OF BORDETELLA-PERTUSSIS
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DOI：
10.1128/iai.60.4.1302-1313.1992
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发表时间：
1992-04-01
影响因子：
3.1
通讯作者：
LEININGER, E
中科院分区：
文献类型：
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作者：
BARENKAMP, SJ;LEININGER, E
流感嗜血杆菌的一组高分子量表面暴露蛋白是人血清抗体（S。J. Barenkamp和F. F. Bodor，Pediatr. Infect. J. 9：333-337，1990）。 为了进一步表征这些蛋白质，我们克隆和测序基因编码两个相关的高分子量蛋白质的原型nontypeable嗜血杆菌菌株。 编码120-kDa嗜血杆菌蛋白的基因由4.4-kbp开放阅读框组成，编码125-kDa嗜血杆菌蛋白的基因由4.6-kbp开放阅读框组成。 这两个基因的前1,259 bp是相同的。 此后，序列开始分化，但总体上它们是80%相同的，并且衍生的氨基酸序列显示70%相同性。 蛋白质序列同源性搜索证明了两个克隆基因的衍生氨基酸序列和编码丝状血凝素的基因的衍生氨基酸序列之间的相似性，丝状血凝素是革兰氏阴性病原体百日咳博德特氏菌产生的表面蛋白。 针对由4.6 kbp开放阅读框编码的重组蛋白产生的抗血清识别原型菌株中的120和125 kDa蛋白，以及125个流行病学上无关的不可分型的H.流感病毒株。 针对重组蛋白的抗血清也识别纯化的丝状血凝素。 丝状血凝素的鼠单克隆抗体识别原型菌株中的120-kDa和125-kDa蛋白，以及与35%的不可分型H.流感菌株收集。 因此，我们已经确定和部分特点的一组高度免疫原性的表面暴露的蛋白质的nontypeable H。与B的丝状血凝素相关的流感病毒。百日咳。
A group of high-molecular-weight surface-exposed proteins of nontypeable Haemophilus influenzae are major targets of human serum antibody (S. J. Barenkamp and F. F. Bodor, Pediatr. Infect. Dis. J. 9:333-337, 1990). To further characterize these proteins, we cloned and sequenced genes encoding two related high-molecular-weight proteins from a prototype nontypeable Haemophilus strain. The gene encoding a 120-kDa Haemophilus protein consisted of a 4.4-kbp open reading frame, and the gene encoding a 125-kDa protein consisted of a 4.6-kbp open reading frame. The first 1,259 bp of the two genes were identical. Thereafter, the sequences began to diverge, but overall they were 80% identical, and the derived amino acid sequences showed 70% identity. A protein sequence homology search demonstrated similarity between the derived amino acid sequences of both cloned genes and the derived amino acid sequence of the gene encoding filamentous hemagglutinin, a surface protein produced by the gram-negative pathogen Bordetella pertussis. Antiserum raised against a recombinant protein encoded by the 4.6-kbp open reading frame recognized both the 120- and the 125-kDa proteins in the prototype strain as well as antigenically related high-molecular-weight proteins in 75% of a collection of 125 epidemiologically unrelated nontypeable H. influenzae strains. The antiserum directed against the recombinant protein also recognized purified filamentous hemagglutinin. A murine monoclonal antibody to filamentous hemagglutinin recognized both the 120-kDa and the 125-kDa protein in the prototype strain as well as proteins identical to those recognized by the recombinant-protein antiserum in 35% of the nontypeable H. influenzae strain collection. Thus, we have identified and partially characterized a group of highly immunogenic surface-exposed proteins of nontypeable H. influenzae which are related to the filamentous hemagglutinin of B. pertussis.