Establishment of a murine model of bone invasion by oral squamous cell carcinoma
Establishment of a murine model of bone invasion by oral squamous cell carcinoma
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DOI：
10.1016/j.oraloncology.2006.03.015
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发表时间：
2007-03-01
期刊：
影响因子：
4.8
通讯作者：
Takano, Nobuo
中科院分区：
文献类型：
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作者：
Nomura, Takeshi;Shibahara, Takahiko;Takano, Nobuo
本研究旨在建立口腔鳞癌骨侵袭的动物模型，探讨破骨细胞介导的骨侵袭机制。将SCCVII细胞接种到C3 H/HeN小鼠的咬肌区域中。在第3周结束时，处死所有存活小鼠，并通过使用显微计算机断层扫描的三维成像、使用苏木精-伊红染色和抗酒石酸酸性磷酸酶染色的组织病理学观察以及破骨细胞相关细胞因子IL-6、TNF-α和PTHrP的mRNA表达的确认进行分析。SCCVII细胞在小鼠的咬肌中迅速繁殖。骨侵袭仅在SCCVII移植组的显微计算机断层扫描上明显。用H-E和TRAP染色获得的组织病理学结果表明，SCCVII移植组的所有动物的下颌骨中的肿瘤细胞表现出索状侵袭，并呈现锯齿状的骨吸收模式。IL-6、PTHrP和TNF-α的mRNA表达随着对照的降低而增加。SCCVII细胞在C3 H/HeN小鼠的下颌骨中具有高度侵袭性。该模型类似于人口腔癌侵袭上颌骨和下颌骨。我们的下颌骨侵犯模型可能为口腔癌骨侵犯的诊断和治疗提供一个强有力的新模式。(c)2006爱思唯尔有限公司保留所有权利。
This study examines the establishment an animal model of bone invasion by oral squamous cell carcinoma to clarify the mechanisms of osteoclast-mediated bone invasion. C3H/HeN mice were inoculated with SCCVII cells into the masseter region. At the end of week 3, all surviving mice were sacrificed and analyzed by three-dimensional imaging using microcomputed tomography, histopathological observation using Hematoxytin-Eosin staining and Tartrate-Resistant Acid Phosphatase staining, and confirmation of mRNA expression of the osteoclast-related cytokines IL-6, TNF-alpha, and PTHrP. SCCVII cells rapidly multiplied in the masseter muscle of the mice. Bone invasion was evident only in the SCCVII transplanted group on micro-computed tomography. The histopathologic findings obtained with H-E and TRAP staining indicated that the tumor cells in the mandible of all animals of the SCCVII transplanted group exhibited funicular invasion and presented a serrated pattern of bone resorption. The mRNA expression of IL-6, PTHrP, and TNF-alpha increased as the control decreased. SCCVII cells were highly invasive into mandibular bone in C3H/HeN mice. This model was similar to the invasion of human oral cancer into maxillary and mandibular bone. Our mandibular invasion model may provide a powerful new modality for the diagnosis and treatment of oral cancer with bone invasion. (c) 2006 Elsevier Ltd. All rights reserved.