Cis-urocanic acid increases immunotoxicity and lethality of dermally administered permethrin in C57BL/6N mice.
Cis-urocanic acid increases immunotoxicity and lethality of dermally administered permethrin in C57BL/6N mice.
复制标题
顺式尿刊酸可增加 C57BL/6N 小鼠经皮注射氯菊酯的免疫毒性和致死率。
DOI：
10.1080/10915810305070
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发表时间：
2003
影响因子：
2.2
通讯作者：
Holladay,SD
中科院分区：
文献类型：
--
作者：
Prater,MR;GogalJr,RM;Blaylock,BL;Holladay,SD
在 4 至 5 周龄雌性 C57BL/6N 小鼠中评估了单次局部氯菊酯暴露、5 天暴露顺式尿刊酸 (c UCA) 或两种化学物质组合的免疫调节作用。单独使用氯菊酯可降低胸腺重量和细胞结构。虽然单独使用 c UCA 不会影响胸腺终点，但同时暴露于外用氯菊酯和 c UCA 会加剧氯菊酯的胸腺溶解作用。单次局部剂量的氯菊酯还抑制了分离的脾白细胞的几种免疫反应。这包括脾 T 细胞对有丝分裂原的增殖反应、脾巨噬细胞过氧化氢的产生以及脾 B 淋巴细胞特异性抗体的产生。与同时暴露于这些药物对胸腺终点的影响不同，c UCA 不会加剧氯菊酯对任何检查的脾脏终点的不利影响。这些结果似乎表明这些化合物影响前体和功能成熟 T 细胞的不同机制。在本研究中使用的剂量下，氯菊酯对部分小鼠造成了神经毒性作用，包括致死性。由于不明原因，c UCA 显着增加了氯菊酯引起的致死率。尽管本研究中使用的氯菊酯剂量超过了人类医学中通常使用的剂量，但这些结果引起了一些担忧，即阳光通过 c UCA 可能会增加单独使用氯菊酯引起的中枢神经系统不良和免疫影响的风险。
Immunomodulatory effects of a single topical permethrin exposure, 5-day exposure tocis-urocanic acid (c UCA), or a combination of the two chemicals were evaluated in 4- to 5-week-old female C57BL/6N mice. Permethrin alone decreased thymic weight and cellularity. Although c UCA alone did not affect thymic end points, coexposure to topical permethrin and c UCA exacerbated the thymolytic effects of permethrin. The single topical dose of permethrin also depressed several immune responses in isolated splenic leukocytes. This included splenic T-cell proliferative response to mitogen, splenic macrophage hydrogen peroxide production, and splenic B lymphocyte-specific antibody production. Unlike the effect of coexposure to these agents on thymic end points, c UCA did not exacerbate permethrin's adverse effect on any of the splenic end points examined. These results appear to suggest divergent mechanisms by which these compounds affect precursor and functionally mature T cells. At the doses used in this study, permethrin caused neurotoxic effects, including lethality, in a portion of the mice. For undetermined reasons, c UCA significantly increased the rate of lethality caused by permethrin. Although the permethrin doses used in this study exceed that typically used in human medicine, these results raise some concerns about the possibility that sunlight, via c UCA, may increase the risk of adverse central nervous system and immune effects caused by permethrin alone.