A SIMPLE METHOD FOR ARTIFICIAL INFECTION OF TSETSE, GLOSSINA-MORSITANS-MORSITANS LARVAE WITH THE DNA VIRUS OF GLOSSINA-PALLIDIPES
A SIMPLE METHOD FOR ARTIFICIAL INFECTION OF TSETSE, GLOSSINA-MORSITANS-MORSITANS LARVAE WITH THE DNA VIRUS OF GLOSSINA-PALLIDIPES
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DOI：
10.1017/s1742758400014909
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发表时间：
1993-06-01
期刊：
影响因子：
--
通讯作者：
OTIENO, LH
中科院分区：
文献类型：
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作者：
JURA, WGZO;ZDAREK, J;OTIENO, LH
将新保藏的Glossina morsitans morsitans幼虫在冰上冷却，并用1穆尔来自Glossina pallidipes唾液腺匀浆的病毒悬浮液或无菌采采蝇生理盐水接种。让它们化蛹，然后保持在25 ℃，70% r.h.直到羽化后不久，检查唾液腺是否增大和是否存在病毒颗粒。特纳拉尔湾M.作为幼虫接受病毒接种物的Morsitans（n = 135）全部被感染，如通过它们的唾液腺的大体肥大和腺上皮细胞和管腔内病毒颗粒的超微结构表现所揭示的。在对照组中，接受采采蝇生理盐水（n = 91），只有1.1%的苍蝇表现出唾液腺肿大，这一水平相当于在G. Morsitans殖民地。该技术为测试该病毒的生物防治潜力开辟了道路。从G. pallidipes对G. morsitans morsitans，表明在各种Glossina属中报道的肥大的白垩色唾液腺，都是同一种病毒感染的表现
Newly deposited Glossina morsitans morsitans larvae were chilled over ice and inoculated with 1 mul of either virus suspension derived from Glossina pallidipes salivary gland homogenate or sterile tsetse physiological saline. They were allowed to pupariate and then maintained at 25-degrees-C, 70% r.h. until soon after emergence when their salivary glands were examined for enlargement and presence of virus particles. Teneral G. m. morsitans which received the virus inoculum (n = 135) as larvae all became infected as revealed by gross hypertrophy of their salivary glands and ultrastructural manifestation of virus particles within the glandular epithelial cells and lumina. In the control group, which received the tsetse physiological saline (n = 91), only 1.1% of the flies showed the salivary gland enlargement, a level equivalent to the prevalence of virus infection normally detectable in the G. morsitans colony. This technique opens the way for testing the biocontrol potential of this virus. The DNA virus from G. pallidipes is clearly infective to G. morsitans morsitans, suggesting that the hypertrophied, chalky-white salivary glands, reported in various Glossina spp., are a manifestation of infection by one and the same virus.