Shotgun cholanomics of ileal fluid.
Shotgun cholanomics of ileal fluid.
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DOI：
10.1016/j.biochi.2012.09.004
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发表时间：
2013-03
期刊：
影响因子：
3.9
通讯作者：
Griffiths WJ
中科院分区：
文献类型：
--
作者：
Chen Y;Ogundare M;Williams CM;Wang Y;Wang Y;Sewell GW;Smith PJ;Rahman FZ;O'Shea N;Segal AW;Griffiths WJ
本研究建立了一种快速测定肠液中胆汁酸的鸟枪法。该方法是半定量的，并且需要很少的样品制备。胆盐可能参与克罗恩病的发病机制。在一项初步研究中，我们通过分析7名克罗恩病患者和3名健康对照的回肠液的胆汁酸含量来证明该方法。观察到的主要胆汁酸为二和/或三羟基胆酸盐、二和/或三羟基胆酰甘氨酸、二和/或三羟基胆酰牛磺酸、单硫酸化二羟基胆酸盐和单硫酸化二羟基胆酰甘氨酸。该方法可以类似地应用于来源于肠的其他部分的样品。
In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn’s disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn’s disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or tri-hydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.