HORMONAL-CONTROL OF VITELLOGENESIS IN THE GYPSY-MOTH, LYMANTRIA-DISPAR (L) - SUPPRESSION OF HEMOLYMPH VITELLOGENIN BY THE JUVENILE-HORMONE ANALOG, METHOPRENE
HORMONAL-CONTROL OF VITELLOGENESIS IN THE GYPSY-MOTH, LYMANTRIA-DISPAR (L) - SUPPRESSION OF HEMOLYMPH VITELLOGENIN BY THE JUVENILE-HORMONE ANALOG, METHOPRENE
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DOI：
10.1016/0022-1910(90)90107-q
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发表时间：
1990-01-01
影响因子：
2.2
通讯作者：
BORKOVEC, AB
中科院分区：
文献类型：
--
作者：
DAVIS, RE;KELLY, TJ;BORKOVEC, AB
在大多数昆虫中，雌性特异性卵蛋白前体（卵黄原蛋白）在蛹或成虫阶段响应于20-羟基蜕皮激素和/或保幼激素而合成。在舞毒蛾，两个女性特异性的多肽（apo-vitellogenins）具有电泳和免疫学身份与舞毒蛾卵卵黄脱辅基蛋白开始积累在血淋巴后3天的第五龄幼虫。头部结扎、饥饿或在第3天之前用> 10 μ g剂量的保幼激素类似物甲氧普烯处理阻断血淋巴中脱辅基卵黄蛋白原的积累。与头部结扎或饥饿的幼虫不同，处理后体重不会增加，也不会积累正常水平的非卵黄蛋白原血淋巴蛋白，甲氧普烯处理的幼虫正常生长并积累非卵黄蛋白原血淋巴蛋白。20-羟基蜕皮激素（2.5 μ g）对正常幼虫中脱辅基卵黄蛋白原的血淋巴积累没有影响，并且不能恢复甲氧普仑处理的、头部结扎的或饥饿的幼虫中的血淋巴脱辅基卵黄蛋白原。上述结果表明，舞毒蛾血淋巴中卵黄蛋白原的积累需要在幼虫末龄早期（3天前）的关键阶段保持低的保幼激素滴度（或下降）。其他因素，如营养摄入量也可能参与其中。保幼激素作为卵黄蛋白原积累/合成的抑制剂的这种作用对于昆虫物种是一种新的作用。
In most insects, female-specific egg-protein procursors (vitellogenins) are synthesized during pupal or adult stages in response to 20-hydroxyecdysone and/or juvenile hormone. In the gypsy moth, two female-specific polypeptides (apo-vitellogenins) having electrophoretic and immunologic identity with Lymantria dispar egg-vitellin apo-proteins begin to accumulate in the haemolymph after day 3 of the fifth larval instar. Head ligation, starvation or treatment with doses of a juvenile hormone analogue, methoprene, > 10 .mu.g prior to day 3 block the accumulation of apo-vitellogenin in the haemolymph. Unlike head-ligated or starved larvae which do not gain weight and do not accumulate normal levels of non-vitellogenin haemolymph proteins following treatment, methoprene-treated larvae grow normally and accumulate non-vitellogenin haemolymph proteins. 20-Hydroxyecdysone (2.5 .mu.g) has no effect on the haemolymph accumulation of apo-vitellogenins in normal larvae, and does not restore haemolymph apo-vitellogenins in methoprene-treated, head-ligated or starved larvae. The above results subbest that a low (or declining) juvenile hormone titre during a critical stage early in the last larval instar (prior to day 3) is necessary for the accumulation of vitellogenin inthehaemolymph to proceed normally in the gypsy moth. Other factors such as nutrient intake may also be involved. This role of juvenile hormone as an inhibitor of vitellogenin accumulation/synthesis is a novel one for insect species.