Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces
Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces
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DOI：
10.1098/rsif.2004.0019
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发表时间：
2005-03-22
影响因子：
3.9
通讯作者：
Donahue, HJ
中科院分区：
文献类型：
--
作者：
Lim, JY;Hansen, JC;Donahue, HJ
纳米尺度细胞-基质相互作用在各种生物医学应用中具有重要意义。我们研究了人胎儿成骨细胞的反应随机分布的纳米岛地形与不同的高度（11，38和85 nm）产生的聚苯乙烯（PS）/聚溴苯乙烯聚合物分层技术。细胞显示岛状符合板状伪足传播，和丝状伪足的预测似乎发挥了作用，在传感的纳米地形。在11 nm高的岛上培养的细胞显示出比较大纳米岛或平坦PS对照上的细胞显著增强的细胞铺展和更大的细胞尺寸，在较大纳米岛或平坦PS对照上的细胞通常显示出星形形状。信号传递结构，如局灶性粘着斑蛋白和细胞骨架肌动蛋白应力纤维的发展更为明显。正如它们在细胞培养油中的共定位一样，更小的纳米岛表面。细胞粘附和增殖随着岛高度的降低而增加。碱性磷酸酶（AP）活性，骨细胞分化的所有早期标志物。也表现出纳米形貌依赖性，即与较大的岛或平坦的PS相比，11 nm岛上的AP活性更高。因此，具有不同纳米级高度的随机分布的岛状形貌不仅影响粘附相关的细胞行为，而且影响骨细胞表型。我们的研究结果表明，调制的纳米形貌可以利用控制细胞功能的细胞生物材料界面。
Nanoscale cell-substratum interactions are of significant interest in various biomedical applications. We investigated human foetal osteoblastic cell response to randomly distributed nanoisland topography with varying heights (11, 38 and 85 nm) produced by a polystyrene (PS)/polybromostyrene polymer-demixing technique. Cells displayed island-conforming lamellipodia spreading, and filopodia projections appeared to play a role in sensing the nanotopography. Cells cultured on 11 nm high islands displayed significantly enhanced cell spreading and larger cell dimensions than cells oil larger nanoislands or flat PS control, on which cells often displayed a stellate shape. Development of signal transmitting structures such as focal adhesive vinculin protein and cytoskeletal actin stress fibres was more pronounced. as was their colocalization, in cells cultured oil smaller nanoisland surfaces. Cell adhesion and proliferation were greater with decreasing island height. Alkaline phosphatase (AP) activity, all early stage marker of bone cell differentiation. also exhibited nanotopography dependence, i.e. higher AP activity on 11 nm islands compared with that on larger islands or flat PS. Therefore, randomly distributed island topography with varying nanoscale heights not only affect adhesion-related cell behaviour but also bone cell phenotype. Our results suggest that modulation of nanoscale topography may be exploited to control cell function at cell biomaterial interfaces.