Parabrachial complex processes dura inputs through a direct trigeminal ganglion-to-parabrachial connection.
Parabrachial complex processes dura inputs through a direct trigeminal ganglion-to-parabrachial connection.
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DOI：
10.1016/j.ynpai.2021.100060
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发表时间：
2021-01
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影响因子：
--
通讯作者：
Keller A
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文献类型：
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作者：
Uddin O;Anderson M;Smith J;Masri R;Keller A
臂旁复合体（PB）神经元对硬脑膜刺激有可靠的反应。三叉神经节（TG）神经元直接投射到PB。TG神经元同时投射到硬脑膜和脑脊膜，表明两者之间存在直接联系。偏头痛会导致严重的残疾，并在很大程度上增加了医疗费用。脑膜最外层（硬脑膜）的刺激和三叉神经节的激活有助于偏头痛的开始。中枢疼痛处理区域的不适应变化对维持疼痛也很重要。臂旁复合体（PB）是介导慢性疼痛的中枢区域。PB接收多种感觉信息，包括来自三叉神经节的直接输入。我们假设PB处理来自硬脑膜的输入。通过麻醉大鼠单个单元的体内电生理记录，我们发现58个外侧脑脊膜神经元对硬脑膜电刺激反应可靠且潜伏期短。在PB内注射示踪剂后，解剖检查显示三叉神经节内逆行标记的细胞体。神经解剖束迹显示三叉神经节中有一群神经元支配硬脑膜并直接投射到脑脊膜。这些发现表明，脑脊液在处理硬脑膜输入方面具有重要作用，并表明它直接参与偏头痛的发病机制。
Parabrachial complex (PB) neurons respond reliably to dura stimulation. Trigeminal ganglion (TG) neurons project directly to PB. TG neurons project to both dura and PB, demonstrating a direct connection. Migraines cause significant disability and contribute heavily to healthcare costs. Irritation of the meninges’ outermost layer (the dura mater), and trigeminal ganglion activation contribute to migraine initiation. Maladaptive changes in central pain-processing regions are also important in maintaining pain. The parabrachial complex (PB) is a central region that mediates chronic pain. PB receives diverse sensory information, including a direct input from the trigeminal ganglion. We hypothesized that PB processes inputs from the dura. Using in vivo electrophysiology recordings from single units in anesthetized rats we identified 58 neurons in lateral PB that respond reliably and with short latency to electrical dura stimulation. After injecting tracer into PB, anatomical examination reveals retrogradely labeled cell bodies in the trigeminal ganglion. Neuroanatomical tract-tracing revealed a population of neurons in the trigeminal ganglion that innervate the dura and project directly to PB. These findings indicate that PB is strategically placed to process dura inputs and suggest that it is directly involved in the pathogenesis of migraine headaches.