NEUROPEPTIDE-CONTAINING NEURONS OF THE CEREBRAL-CORTEX ARE ALSO GABAERGIC
NEUROPEPTIDE-CONTAINING NEURONS OF THE CEREBRAL-CORTEX ARE ALSO GABAERGIC
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DOI：
10.1073/pnas.81.20.6526
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
EMSON, PC
中科院分区：
文献类型：
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作者：
HENDRY, SHC;JONES, EG;EMSON, PC
对猫和猴大脑皮层中的神经元进行免疫细胞化学染色，检测谷氨酸脱羧酶（GluDCase；L-谷氨酸 1-羧基裂解酶，EC 4.1.1.15）、生长抑素（SRIF）、神经肽 Y（NPY）和缩胆囊素八肽（CCK）。在检查的所有皮层区域（躯体感觉、运动顶叶和视觉区域）中，对这些分子中的每一种分子表现出免疫反应性的神经元都是非锥体细胞。通过抗体洗脱方法证明了 GluDCase 免疫反应性与肽免疫反应性在相同细胞中的共定位，通过免疫荧光对相同细胞进行染色，首先对肽进行染色，然后对 GluDCase 进行染色；用羊抗GluDase和兔抗肽抗血清对相同切片进行双重染色，结合的抗体由罗丹明缀合的驴抗羊和荧光素缀合的猪抗兔二抗血清定位。通过这两种方法，在检查的皮层的所有区域中都发现了对 GluDCase 和每种肽具有免疫反应的细胞体。通过单切片双标记，发现猫皮质中所有CCK、SRIF和NPY免疫反应性细胞以及猴皮质中90%-95%的细胞也呈GluDCase阳性。更多的细胞仅对 GluDase 产生免疫反应。 GluDCase 不仅与 CCK、SRIF 或 NPY 共定位于细胞体中，而且还定位于小点状结构中，这些结构可能是轴突末端。从之前的 EM 研究中获得的数据来看，表现出 GluDCase 和 CCK 样免疫反应性的神经元可能与表现出 GluDCase 和 SRIF 样免疫反应性的神经元不同。 NPY 与 SRIF 免疫反应性共定位。皮质中间神经元类别包含传统神经递质 GABA 和神经调节剂（肽之一）。
Neurons in the cat and monkey cerebral cortex were stained immuncytochemically for glutamic acid decarboxylase (GluDCase; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), somatostatin (SRIF), neuropeptide Y (NPY) and cholecystokinin octapeptide (CCK). In all areas of cortex examined (somatic sensory, motor parietal and visual areas), neurons displaying immunoreactivity for each of these molecules were nonpyramidal cells. Co-localization of GluDCase immunoreactivity with peptide immunoreactivity in the same cells was demonstrated by the antibody elution method, staining the same cells by immunofluorescence, first for a peptide and then for GluDCase; double staining of the same sections with sheep anti-GluDCase and rabbit anti-peptide antisera, the bound antibodies being localized by rhodamine-conjugated donkey anti-sheep and fluorescein-conjugated swine anti-rabbit secondary antisera. With both procedures, cell bodies immunoreactive for GluDCase and for each of the peptides were found in all areas of cortex examined. With double labeling on single sections, it was found that all CCK-, SRIF- and NPY-immunoreactive cells in cat cortex and 90%-95% in monkey cortex are also GluDCase positive. Many more cells are immunoreactive for GluDCase alone. GluDCase was co-localized with CCK, SRIF or NPY not only in cell somata, but also in small punctate structures, which are likely to be axon terminals. From the data gained in previous EM studies, neurons displaying GluDCase-and CCK-like immunoreactivity are probably a class separate from those displaying GluDCase- and SRIF-like immunoreactivity. NPY is co-localized with SRIF immunoreactivity. Classes of cortical interneuron contain a conventional neurotransmitter GABA and a neureomodulator (one of the peptides).