Cell proliferation by silk gut incorporating FGF-2 protein microcrystals.
Cell proliferation by silk gut incorporating FGF-2 protein microcrystals.
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DOI：
10.1038/srep11051
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发表时间：
2015-06-08
影响因子：
4.6
通讯作者：
Mori H
中科院分区：
文献类型：
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作者：
Kotani E;Yamamoto N;Kobayashi I;Uchino K;Muto S;Ijiri H;Shimabukuro J;Tamura T;Sezutsu H;Mori H
由家蚕丝腺加工而成的丝肠是一种理想的细胞生长因子可生物降解载体。我们以前证明，多角体，微晶体的Cypovirus 1多角体蛋白，可以作为多功能载体蛋白。在这里，我们报告了一种转基因家蚕的产生，表达多角体蛋白与人碱性成纤维细胞生长因子（FGF-2）在其后部丝腺利用丝肠作为蛋白质载体，以保护和缓慢释放活性细胞生长因子。在后部丝腺中，多面体蛋白形成多面体微晶，并且FGF-2由于多面体固定信号而被封装在多面体内。从后部丝腺制备的丝肠粉含有多角体包裹的FGF-2，刺激p44/p42 MAP激酶的磷酸化，并通过释放生物活性FGF-2诱导血清饥饿的NIH 3 T3细胞增殖。即使在25 °C下孵育一周后，相对于具有未包封的FGF-2的丝肠粉末，观察到掺入多面体包封的FGF-2的丝肠粉末的FGF-2的显著更高的生物活性。我们的研究结果表明，后丝腺纳入多面体封装的FGF-2适用于制备可生物降解的丝肠，它可以保护和释放FGF-2，在无病毒和无血清的表达系统中产生具有显着的应用潜力。
Silk gut processed from the silk glands of the silkworm could be an ideal biodegradable carrier for cell growth factors. We previously demonstrated that polyhedra, microcrystals of Cypovirus 1 polyhedrin, can serve as versatile carrier proteins. Here, we report the generation of a transgenic silkworm that expresses polyhedrin together with human basic fibroblast growth factor (FGF-2) in its posterior silk glands to utilize silk gut as a proteinaceous carrier to protect and slowly release active cell growth factors. In the posterior silk glands, polyhedrin formed polyhedral microcrystals, and FGF-2 became encapsulated within the polyhedra due to a polyhedron-immobilization signal. Silk gut powder prepared from posterior silk glands containing polyhedron-encapsulated FGF-2 stimulated the phosphorylation of p44/p42 MAP kinase and induced the proliferation of serum-starved NIH3T3 cells by releasing bioactive FGF-2. Even after a one-week incubation at 25 °C, significantly higher biological activity of FGF-2 was observed for silk gut powder incorporating polyhedron-encapsulated FGF-2 relative to silk gut powder with non-encapsulated FGF-2. Our results demonstrate that posterior silk glands incorporating polyhedron-encapsulated FGF-2 are applicable to the preparation of biodegradable silk gut, which can protect and release FGF-2 that is produced in a virus- and serum-free expression system with significant application potential.
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DOI：
10.1534/g3.114.011643
发表时间：
2014-05-28
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Tsubota T;Uchino K;Suzuki TK;Tanaka H;Kayukawa T;Shinoda T;Sezutsu H
通讯作者：
Sezutsu H
影响因子：
14
作者：
Ijiri, Hiroshi;Coulibaly, Fasseli;Mori, Hajime
通讯作者：
Mori, Hajime
影响因子：
2.9
作者：
Pritchard EM;Dennis PB;Omenetto F;Naik RR;Kaplan DL
通讯作者：
Kaplan DL
影响因子：
5.3
作者：
MacKeigan, JP;Murphy, LO;Blenis, J
通讯作者：
Blenis, J
DOI：
10.1038/nrd2792
发表时间：
2009-03
期刊：
Nature reviews. Drug discovery
影响因子：
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作者：
通讯作者：
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