Systematic evaluation of graphene quantum dot toxicity to male mouse sexual behaviors, reproductive and offspring health
Systematic evaluation of graphene quantum dot toxicity to male mouse sexual behaviors, reproductive and offspring health
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系统评价石墨烯量子点对雄性小鼠性行为、生殖和后代健康的毒性
DOI：
10.1016/j.biomaterials.2018.12.001
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发表时间：
2019
期刊：
影响因子：
14
通讯作者：
Chu Maoquan
中科院分区：
文献类型：
--
作者：
Zhang Ding;Zhang Zefei;Wu Ying;Fu Kai;Chen Yang;Li Wenhao;Chu Maoquan
石墨烯量子点（GQD）在多个领域引起了相当大的关注，特别是生物医学研究。然而，GQD对哺乳动物生殖和后代健康的影响尚不清楚。在此，我们发现GQD经口灌胃或静脉注射对雄性小鼠的性行为频率和时间没有影响。暴露于GQD的雄性小鼠保留了健康的结构和功能生殖生理学（例如，健康精子的产生和储存，睾丸和附睾中正常总蛋白和关键酶浓度的维持，以及正常睾酮水平。与GQD暴露的雄性动物一起饲养的雌性小鼠产生了第一、第二和随后的健康幼仔窝，与与缓冲液处理的雄性动物一起饲养的雌性小鼠无明显差异。这些发现可能是由于GQDs在生殖细胞中的低毒性及其在小鼠中暴露后的快速排泄，主要是尿液和/或粪便; GQDs，即使在高剂量下，在雄性小鼠睾丸、附睾和大脑中几乎检测不到。我们的研究结果揭示了GQD暴露对雄性小鼠性行为、生殖活动和后代发育的短期和长期影响，并指出了GQD的潜在作用机制，以进一步了解其生物安全性。
Graphene quantum dots (GQDs) have attracted considerable attention across multiple fields, particularly biomedical research. However, the effects of GQDs on reproductive and offspring health in mammals are unclear. Here, we show that GQD exposureviaoral gavage or intravenous injection had no effect on the frequency and timing of sexual behaviors in male mice. GQD-exposed male mice retained healthy structural and functional reproductive physiology (e.g., production and storage of healthy sperm, maintenance of normal total protein and key enzyme concentrations in testes) of the testes and epididymides, as well as normal testosterone levels. Female mice housed with GQD-exposed males produced first, second, and subsequent litters of healthy pups without obvious differences to females housed with buffer-treated males. These findings may be explained by the low toxicity of GQDs in germ cells and their rapid excretion after exposure in mice, mainlyviathe urine and/or feces; GQDs, even at high doses, are virtually undetectable in male mouse testis, epididymis, and brain. Our findings reveal the short- and long-term effects of GQD exposure on male mouse sexual behaviors, reproductive activity, and offspring development and indicate the potential mechanisms of action of GQDs to provide further insight into their bio-safety.