Isolation of renal proximal tubular brush-border membranes
Isolation of renal proximal tubular brush-border membranes
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DOI：
10.1038/nprot.2007.156
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Murer, Heini
中科院分区：
文献类型：
--
作者：
Biber, Juerg;Stieger, Bruno;Murer, Heini
该方案描述了一种用于以高产率和高纯度分离和纯化肾近端管状刷状缘膜的方法。基于刷状缘膜与具有二价阳离子（如Mg2+）的其他细胞膜相比的不同反应性，在几个差速离心步骤（约3小时内）后可以获得纯化的膜囊泡，其适合于体外研究，如转运实验或蛋白质和脂质分析。
This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.