Tight protein-DNA interactions favor gene silencing
Tight protein-DNA interactions favor gene silencing
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DOI：
10.1101/gad.611011
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发表时间：
2011-07-01
影响因子：
10.5
通讯作者：
Taddei, Angela
中科院分区：
文献类型：
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作者：
Dubarry, Marion;Loiodice, Isabelle;Taddei, Angela
由酵母沉默信息调节复合物（SIR）形成的异染色质样结构抑制沉默交配型基因座和端粒的转录。在这里，我们报告说，当顺式作用元件在附近时，紧密的蛋白质-DNA 复合物会诱导 SIR 复合物的异位募集，从而促进基因沉默和亚核定位的变化。重要的是，复制叉相关解旋酶 Rrm3 的缺乏增强了这种诱导的基因抑制。此外，Sir3 和 Sir4 在自然复制暂停位点（包括 tRNA 基因）在全基因组范围内富集。一致地，插入 tRNA 基因会促进 SIR 介导的附近基因的沉默。这些结果表明，DNA-蛋白质紧密相互作用产生的复制应激有利于异染色质的形成。
The heterochromatin-like structure formed by the yeast silent information regulator complex (SIR) represses transcription at the silent mating type loci and telomeres. Here, we report that tight protein-DNA complexes induce ectopic recruitment of the SIR complex, promoting gene silencing and changes in subnuclear localization when cis-acting elements are nearby. Importantly, lack of the replication fork-associated helicase Rrm3 enhances this induced gene repression. Additionally, Sir3 and Sir4 are enriched genome-wide at natural replication pause sites, including tRNA genes. Consistently, inserting a tRNA gene promotes SIR-mediated silencing of a nearby gene. These results reveal that replication stress arising fromtight DNA-protein interactions favors heterochromatin formation.