Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.
Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.
复制标题
DOI：
10.3314/jjmm.52.129
复制
发表时间：
2011-01-01
影响因子：
1
通讯作者：
Kanbe, Toshio
中科院分区：
文献类型：
--
作者：
Shimizu, Kazue;Hattori, Hisao;Kanbe, Toshio
本研究的目的是调查白色念珠菌的基因型分布和浅表念珠菌病的主要基因型。C.采用4个微卫星标记（HIS 3、CDC 3、CAI和CA III）对从浅表念珠菌病患者感染部位分离的白色念珠菌（简称感染株）进行片段分析。将感染菌株的基因型与C.从非念珠菌病患者的口腔粘膜分离的白色念珠菌（称为口腔分离株）。C.白念珠菌的HIS 3/CAI基因有4种主要基因型（148：148 / 23：23为a型，148：160 / 33：41为B型，148：164 / 32：41为c型，152：152 / 18：27为d型）。在口腔和感染中，“a“、”B“和“d“基因型分别为4.7%、8.8%和7.6%，分别为6.6%、9.2%和15.4%。从感染部位未分离到基因型“c“的分离株。在生殖器念珠菌病患者分离株中发现了基因型“a“。对来自一个病人的多个分离株进行基因分型，结果表明C。与口腔分离株相比，即使在同一患者中，感染部位的白色念珠菌也具有遗传同源性。
This study aimed to examine the genotype distribution of Candida albicans and the major genotypes involved in superficial candidiasis. The genotypes of C. albicans isolated from the infection sites of patients with superficial candidiasis (referred to as infection isolates) were analyzed by fragment analysis using 4 microsatellite markers (HIS3, CDC3, CAI and CAIII). Genotypes of the infection isolates were compared with those of C. albicans isolated from oral mucosa of non-candidiasis patients (referred to as oral isolates). Isolates of C. albicans showed 4 major genotypes for HIS3/CAI (" a " for 148 : 148 / 23 : 23," b " for 148 : 160 / 33 : 41," c " for 148 : 164 / 32 : 41 and " d " for 152 : 152 / 18 : 27). The genotypes " a "," b " and " d " were commonly found in oral (4.7, 8.8 and 7.6%, respectively) and infection (6.6, 9.2 and 15.4%, respectively) isolates. No isolates of genotype " c " were isolated from infection sites. The genotype " a " was found in the isolates from patients with genitalia candidiasis. Genotyping of multiple isolates from an individual patient showed that C. albicans from infection sites was genetically homogenous as compared with that of oral isolates, even in the same patient with candidiasis.