In vitro method to characterize diffusion of dye from polymeric particles: a model for drug release.
In vitro method to characterize diffusion of dye from polymeric particles: a model for drug release.
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表征染料从聚合物颗粒扩散的体外方法：药物释放模型。
DOI：
10.1021/la900694k
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Pancholi K
中科院分区：
文献类型：
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作者：
Pancholi K
药物递送系统的释放曲线是决定其功效的关键因素。在基于聚合物颗粒的系统的情况下，释放曲线是包括颗粒直径和孔隙率的几个参数的函数。这些参数的影响通常是使用紫外光谱实验研究。为了便于设计更有效的药物递送颗粒，期望预测作为许多参数相互作用的结果的药物从颗粒的释放曲线。在这项工作中，确定的扩散分布的定量方法的开发，消除了重复实验的需要。使用同轴电流体动力雾化制备聚甲基倍半硅氧烷的颗粒，并收集在含有不同浓度的伊文思蓝染料（6、0.6和0.06 mg/mL）的溶液中，所述伊文思蓝染料用于模拟药物。通过求解实验中使用的参数的非稳态扩散方程来计算染料释放曲线。结果表明，在不稳定的二级溶液中，如果用颗粒与液体的比表面积代替周围液体与颗粒的体积比，则可以用一个简单的方程来计算染料从直径为400 nm ~ 9 μm的颗粒中的释放曲线，而不需要增加溶解项。计算结果与实验结果吻合较好，表明该方法可用于确定扩散系数随粒径和材料的变化。这项研究代表了开发完整药物释放模型的关键一步。
The release profile of a drug delivery system is a key factor in determining its efficacy. In the case of a polymeric particle based system, the release profile is a function of several parameters including particle diameter and porosity. The effects of these parameters are usually investigated experimentally using UV-spectroscopy. Predicting the drug release profile from particles as a result of the interaction of many parameters is desirable in order to facilitate the design of more efficient drug delivery particles. In this work, a quantitative method of determining the diffusion profile is developed which removes the need for repetitive experimentation. Particles of polymethylsilsesquioxane were prepared using coaxial electrohydrodynamic atomization and collected in solutions containing different concentrations of Evans blue dye (6, 0.6, and 0.06 mg/mL) which was used to simulate a drug. The dye release profile was calculated by solving the unsteady state diffusion equation for parameters used in the experiments. It was demonstrated that the dye release profile from particles with diameters ranging from 400 nm to 9 μm can be calculated using a simple equation without addition of a dissolution term, if the volume ratio of surrounding liquid to particle in the unsteady second order solution is substituted by the surface area of particles to liquid volume ratio. The calculated data are found to be in good agreement with the experimental, indicating that this method can be used to determine the diffusion coefficient as a function of particle diameter and material. This study represents a crucial step toward developing a full drug release model.