Gonadal steroid hormones maintain the stress-induced acetylcholine release in the hippocampus: Simultaneous measurements of the extracellular acetylcholine and serum corticosterone levels in the same subjects
Gonadal steroid hormones maintain the stress-induced acetylcholine release in the hippocampus: Simultaneous measurements of the extracellular acetylcholine and serum corticosterone levels in the same subjects
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DOI：
10.1210/en.2007-0827
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发表时间：
2008-02-01
期刊：
影响因子：
4.8
通讯作者：
Kimura, Fukuko
中科院分区：
文献类型：
--
作者：
Mitsushima, Dai;Takase, Kenkichi;Kimura, Fukuko
为了检查性腺类固醇激素在海马乙酰胆碱 (ACh) 水平和血清皮质酮水平应激反应中的作用，我们在完整、性腺切除或性腺切除的类固醇引发大鼠中同时观察了这些参数。在两性大鼠中，性腺切除术或性腺类固醇激素替代均不影响乙酰胆碱的基线水平。然而，性腺切除术严重减弱了乙酰胆碱的应激反应，而相应的性腺激素的替代成功地将反应恢复到完整水平。性腺激素以不同的方式影响血清皮质酮水平。睾丸切除大鼠的睾酮替代抑制了皮质酮水平的基线和应激反应，而卵巢切除大鼠的 17 β-雌二醇替代则增加了皮质酮水平。我们进一步发现，给完整雄性大鼠施用来曲唑或氟他胺可减弱乙酰胆碱的应激反应。此外，氟他胺治疗增加了皮质酮的基线水平，而来曲唑治疗减弱了皮质酮的应激反应。此外，我们发现乙酰胆碱水平和皮质酮水平之间存在较低的正相关性，这取决于性腺类固醇激素的存在。我们的结论是：1）性腺类固醇激素维持海马乙酰胆碱水平的应激反应，2）性腺类固醇激素独立调节海马乙酰胆碱和血清皮质酮的应激反应，3）性腺激素对胆碱能应激反应的性别特异性作用可能表明新生儿隔海马胆碱能的性别分化 大鼠体内的系统。
To examine the role of gonadal steroid hormones in the stress responses of acetylcholine (ACh) levels in the hippocampus and serum corticosterone levels, we observed these parameters simultaneously in intact, gonadectomized, or gonadectomized steroid-primed rats. In both sexes of rats, neither gonadectomy nor the replacement of gonadal steroid hormone affected the baseline levels of ACh. However, gonadectomy severely attenuated the stress response of ACh, whereas the replacement of corresponding gonadal hormone successfully restored the response to intact levels. The gonadal hormones affected the serum corticosterone levels in a different manner; the testosterone replacement in orchidectomized rats suppressed the baseline and the stress response of corticosterone levels, whereas the 17 beta-estradiol replacement in ovariectomized rats increased the levels. We further found that letrozole or flutamide administration in intact male rats attenuated the stress response of ACh. In addition, flutamide treatment increased the baseline levels of corticosterone, whereas letrozole treatment attenuated the stress response of corticosterone. Moreover, we found a low positive correlation between the ACh levels and corticosterone levels, depending on the presence of gonadal steroid hormone. We conclude that: 1) gonadal steroid hormones maintain the stress response of ACh levels in the hippocampus, 2) the gonadal steroid hormone independently regulates the stress response of ACh in the hippocampus and serum corticosterone, and 3) the sex-specific action of gonadal hormone on the cholinergic stress response may suggest a neonatal sexual differentiation of the septohippocampal cholinergic system in rats.