Significance of alpha smooth muscle actin expression in traumatic painful neuromas: a pilot study in rats.
Significance of alpha smooth muscle actin expression in traumatic painful neuromas: a pilot study in rats.
复制标题
创伤性疼痛神经瘤中α平滑肌肌动蛋白表达的意义：大鼠初步研究
DOI：
10.1038/srep23828
复制
发表时间：
2016-03-29
影响因子：
4.6
通讯作者：
Yan H
中科院分区：
文献类型：
--
作者：
Weng W;Zhao B;Lin D;Gao W;Li Z;Yan H
疼痛性神经瘤的治疗仍然是一个挑战，神经瘤相关疼痛的机制尚未完全了解。在本研究中，我们旨在观察创伤性神经瘤中α平滑肌肌动蛋白（α-SMA）的表达，并探讨其在大鼠模型神经病理性疼痛病因中的可能作用。使用大鼠坐骨神经，实验分为两部分。在第一部分中，我们的结果显示，自切组的 α-SMA 和疼痛标记物 c-fos 水平显着高于非自切组。在第二部分中，分别使用 SB-431542 和 GW9662 下调和上调神经瘤中 α-SMA 的表达。发现自切评分与α-SMA表达水平之间存在显着相关性（R = 0.957；p < 0.001），并且α-SMA表达水平与自切评分呈正相关（R2 = 0.915，p < 0.001）。我们得出的结论是，α-SMA 的表达在神经瘤相关疼痛中发挥一定作用，或者作为疼痛的直接原因，或者作为局部机械刺激存在的间接标志。我们的研究结果可能为开发治疗顽固性疼痛性神经瘤的新治疗方式提供新的见解。
Treatment of painful neuromas remains a challenge and the mechanism of neuroma-associated pain is not yet fully understood. In this study, we aimed to observe the expression of alpha smooth muscle actin (α-SMA) in traumatic neuromas and to investigate its possible roles in the cause of neuropathic pain in a rat model. The rat sciatic nerve was used and the experiment was divided into two parts. In part I, our results showed significantly higher levels of α-SMA and the pain marker c-fos in the autotomy group than in the no-autotomy group. In part II, the expression of α-SMA in neuromas was down- and up-regulated using SB-431542 and GW9662, respectively. A significant correlation between autotomy scores and the expression level of α-SMA was found (R = 0.957; p < 0.001) and the expression level of α-SMA was positively related to the autotomy scores (R2 = 0.915, p < 0.001). We concluded that the expression of α-SMA plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli. Our findings may provide new insights into the development of new treatment modalities for the management of intractable painful neuromas.