Swab materials and Bacillus anthracis spore recovery from nonporous surfaces.
Swab materials and Bacillus anthracis spore recovery from nonporous surfaces.
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DOI：
10.3201/eid1006.030716
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发表时间：
2004-06
影响因子：
11.8
通讯作者：
Srduino MJ
中科院分区：
文献类型：
--
作者：
Rose L;Jensen B;Peterson A;Banerjee SN;Srduino MJ
评价了四种拭子材料从钢试样中回收炭疽芽孢杆菌孢子的效率。使用棉、大泡沫、聚酯和人造丝拭子对接种有已知浓度孢子悬浮液的试样进行取样。评价了从拭子中去除孢子的三种处理方法（涡旋、超声或轻微搅拌）和两种拭子制备物（预湿和干燥）。结果表明，预湿拭子比干拭子更有效地回收孢子（14.3% vs. 4.4%）。与超声处理拭子12 min或对拭子进行最小搅拌相比，在处理过程中涡旋拭子2 min可获得上级孢子浸提。在处理过程中涡旋的预湿润大泡沫和棉签回收的孢子比例最高，平均回收率分别为43.6%（标准差[SD] 11.1%）和41.7%（SD 14.6%）。预湿润和涡旋的聚酯和人造丝拭子的效率较低，分别为9.9%（SD 3.8%）和11.5%（SD 7.9%）。
Four swab materials were evaluated for their efficiency in recovery of Bacillus anthracis spores from steel coupons. Cotton, macrofoam, polyester, and rayon swabs were used to sample coupons inoculated with a spore suspension of known concentration. Three methods of processing for the removal of spores from the swabs (vortexing, sonication, or minimal agitation) and two swab preparations (premoistened and dry) were evaluated. Results indicated that premoistened swabs were more efficient at recovering spores than dry swabs (14.3% vs. 4.4%). Vortexing swabs for 2 min during processing resulted in superior extraction of spores when compared to sonicating them for 12 min or subjecting them to minimal agitation. Premoistened macrofoam and cotton swabs that were vortexed during processing recovered the greatest proportions of spores with a mean recovery of 43.6% (standard deviation [SD] 11.1%) and 41.7% (SD 14.6%), respectively. Premoistened and vortexed polyester and rayon swabs were less efficient, at 9.9% (SD 3.8%) and 11.5% (SD 7.9%), respectively.