Fabrication of cultured epithelium using oral mucosal cells and its clinical applications.
Fabrication of cultured epithelium using oral mucosal cells and its clinical applications.
复制标题
口腔粘膜细胞培养上皮的制备及其临床应用。
作者：
K. Hata;M. Ueda
培养的粘膜上皮可以在体外用活的粘膜细胞形成并用作移植材料。本文介绍了我们的粘膜上皮细胞培养方法，并与皮肤上皮细胞培养方法进行了比较。根据我们的研究结果，培养的粘膜上皮比培养的皮肤上皮早形成。此外，培养的粘膜上皮细胞的结构比培养的皮肤上皮细胞的结构保持更长的时间。基于我们在体外研究中的发现，我们将这种培养的粘膜上皮应用于人皮肤和粘膜缺损的重建，并成功地修复了缺损。我们还提出了一个概述的问题有关使用这种方法。
A cultured mucosal epithelium can be formed with living mucosal cells in vitro and used as a graft material. In this article, we described our culture methods for preparation of cultured mucosal epithelium as well as its biological characteristics compared with cultured skin epithelium. According to our results, cultured mucosal epithelium was formed earlier than cultured skin epithelium. Furthermore, the structure of cultured mucosal epithelium was maintained longer than that of cultured skin epithelium. Based on our findings from an in vitro study, we applied this cultured mucosal epithelium to the reconstruction of human skin and mucosal defects and succeeded in repairing the defects. We also present an overview of the problem relevant to the use of such methods.