Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct
Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct
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DOI：
10.1523/jneurosci.04-02-00585.1984
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发表时间：
1984-02
期刊：
影响因子：
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通讯作者：
R. Miller;M. Raff
中科院分区：
文献类型：
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作者：
R. Miller;M. Raff
我们研究了半薄冰冻切片的发展和成年大鼠中枢神经系统（CNS）的间接免疫荧光，以确定抗原表型的原生质和纤维星形胶质细胞。用抗胶质细胞酸性蛋白（GFAP）的抗体鉴定星形胶质细胞，我们发现，绝大多数的纤维星形胶质细胞在成人视神经被标记的单克隆抗体A2 B5，而绝大多数的原浆星形胶质细胞在成人大脑皮层没有。位于成人视神经外周的星形胶质细胞形成胶质细胞界膜，与纤维星形胶质细胞相比，它们更像原生质星形胶质细胞，因为它们与抗GFAP抗血清的应变相对较弱，并且是A2 B5-。在发育中的大鼠视神经中，原生质样星形胶质细胞在第一个纤维星形胶质细胞可以被检测到之前至少一周出现。结合我们先前对发育中大鼠视神经的悬浮液和培养物中星形胶质细胞的观察（Raff，M.C.，急诊室Abney，J. Cohen，R.林赛和M. Noble（1983）J. Neurosci. 3：1289-1300; Raff，M.C.，R.H.米勒和M. Noble（1983）Nature 303：390- 396），这些结果表明，原生质和纤维星形胶质细胞是不同类别的神经胶质细胞，其在抗原表型和发育历史以及在CNS内的形态和位置上不同。
We have studied semithin frozen sections of developing and adult rat central nervous system (CNS) by indirect immunofluorescence in order to determine the antigenic phenotype of protoplasmic and fibrous astrocytes. Using antibodies against glial fibrillary acidic protein (GFAP) to identify astrocytes, we show that the great majority of fibrous astrocytes in adult optic nerve are labeled by the monoclonal antibody A2B5, while the great majority of protoplasmic astrocytes in adult cerebral cortex are not. Astrocytes located at the periphery of the adult optic nerve that form the glial limiting membrane are more like protoplasmic astrocytes than fibrous astrocytes in that they strain relatively weakly with anti-GFAP antiserum and are A2B5-. In the developing rat optic nerve, protoplasmic-like astrocytes appear at least one week before the first fibrous astrocytes can be detected. Taken together with our previous observations on astrocytes in suspensions and cultures of developing rat optic nerve (Raff, M.C., E.R. Abney, J. Cohen, R. Lindsay, and M. Noble (1983) J. Neurosci. 3: 1289–1300; Raff, M.C., R.H. Miller, and M. Noble (1983) Nature 303: 390– 396), these results suggest that protoplasmic and fibrous astrocytes are distinct classes of glial cells that differ in their antigenic phenotype and developmental history, as well as in their morphology and location within the CNS.